Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity
2013 (English)In: Nanoscale, ISSN 2040-3364, Vol. 5, no 19, 8973-8976 p.Article in journal (Refereed) Published
A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of 5 nM (0.1 μg mL−1).
Place, publisher, year, edition, pages
Royal Society of Chemistry , 2013. Vol. 5, no 19, 8973-8976 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-99413DOI: 10.1039/c3nr03006gISI: 000324500900027OAI: oai:DiVA.org:liu-99413DiVA: diva2:656882
Funding Agencies|School of Materials Science and Engineering, Nanyang Technological University, Singapore||Provost Office, Nanyang Technological University, Singapore||Swedish Foundation for Strategic Research (SSF)||Knut and Alice Wallenberg Foundation (KAW), CeNano||Swedish Research Council (VR)||2013-10-172013-10-172014-10-08