A novel 34 kDa glutathione-binding protein in mature rat ovary
1994 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 201, no 1, 149-154 p.Article in journal (Refereed) Published
A novel protein that binds to a glutathione-Sepharose affinity column has been detected in mature, but not immature, rat ovary. This protein could be resolved from all identifiable components when the affinity-purified material, containing primarily glutathione transferases, was analyzed on reversed phase-HPLC. The unidentified protein migrated with an apparent molecular weight of 34 kDa on SDS-PAGE. After purification by affinity chromatography and subsequent preparative electrophoresis, the protein was subjected to N-terminal amino acid sequence analysis. The sequence obtained demonstrated a high degree of homology with an internal amino acid sequence in human carbonyl reductase (EC 126.96.36.199).
Place, publisher, year, edition, pages
Elsevier, 1994. Vol. 201, no 1, 149-154 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-99880DOI: 10.1006/bbrc.1994.1681ISI: A1994NN87700021PubMedID: 8198567OAI: oai:DiVA.org:liu-99880DiVA: diva2:658725