liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Xenografted fetal dorsal root ganglion, embryonic stem cell and adult neural stem cell survival following implantation into the adult vestibulocochlear nerve
Karolinska Hospital, Stockholm, Sweden.
Karolinska Hospital, Stockholm, Sweden.
Karolinska Hospital, Stockholm, Sweden.
Karolinska Institutet, Stockholm, Sweden.
Show others and affiliations
2005 (English)In: Experimental Neurology, ISSN 0014-4886, E-ISSN 1090-2430, Vol. 193, no 2, 326-333 p.Article in journal (Refereed) Published
Abstract [en]

Sensorineural hearing loss is a disabling condition. In the post-embryonic and adult mammalian inner ear, the regeneration of auditory hair cells, spiral ganglion neurons or their axons does not occur naturally. This decrease in excitable neurons limits the success of auditory rehabilitation.

Allografts and xenografts have shown promise in the treatment of a variety of neurological diseases. Fetal dorsal root ganglion (DRG) neurons can extend functional connections in the rat spinal cord. Embryonic stem cells (ES cells) and adult neural stem cells (ANSC) have the potential to differentiate into neurons.

We have implanted embryonic days (E) 13–16 fetal mouse DRGs from transgenic mouse lines that express Enhanced Green Fluorescent Protein (EGFP) or lacZ reporter genes, EGFP-expressing ES cells or lacZ-expressing ANSC into the injured vestibulocochlear nerve of adult rats and guinea pigs. Survival of the implants was assessed 2 to 4 weeks postoperatively. For further evaluation of the differentiation of the implanted ES-cells, we double labeled with the mouse-specific neuronal antibody Thy 1.2.

The rats implanted with EGFP- or lacZ-expressing DRGs showed labeled DRGs after sacrifice. In addition, EGFP-positive nerve fibers were seen growing within the proximal nerve. The results from the EGFP ES cells and lacZ ANSC revealed reporter-expressing cells at the site of injection in the vestibulocochlear nerve of the host rats and guinea pigs but also within the brain stem. Thy 1.2 profiles were seen among the EGFP ES cells within the 8th cranial nerve.

The findings of this study indicate that the vestibulocochlear nerve of adult rats and guinea pigs will support xenotransplants of embryonic DRG, ES cells and ANSC. This may have future clinical applicability in recreating a neuronal conduit following neuronal injury between the inner ear and the central nervous system (CNS).

Place, publisher, year, edition, pages
Elsevier, 2005. Vol. 193, no 2, 326-333 p.
Keyword [en]
hearing; embryonic stein cells; adult neural stein cells; dorsal root ganglia; transplantation; vestibulocochlear nerve; cochlear implant
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-101029DOI: 10.1016/j.expneurol.2004.12.027ISI: 000229134900006PubMedID: 15869935OAI: diva2:665033
Available from: 2013-11-18 Created: 2013-11-18 Last updated: 2013-11-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Olivius, Petri
In the same journal
Experimental Neurology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 26 hits
ReferencesLink to record
Permanent link

Direct link