liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Functional Evaluation of a Cell Replacement Therapy in the Inner Ear
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden.
University of Michigan, Ann Arbor, MI, USA .
Karolinska Institute/Karolinska University Hospital, Stockholm, Sweden .
Show others and affiliations
2009 (English)In: Otology and Neurotology, ISSN 1531-7129, E-ISSN 1537-4505, Vol. 30, no 4, 551-558 p.Article in journal (Refereed) Published
Abstract [en]


Cell replacement therapy in the inner ear will contribute to the functional recovery of hearing loss.


Cell replacement therapy is a potentially powerful approach to replace degenerated or severely damaged spiral ganglion neurons. This study aimed at stimulating the neurite outgrowth of the implanted neurons and enhancing the potential therapeutic of inner ear cell implants.


Chronic electrical stimulation (CES) and exogenous neurotrophic growth factor (NGF) were applied to 46 guinea pigs transplanted with embryonic dorsal root ganglion (DRG) neurons 4 days postdeafening. The animals were evaluated with the electrically evoked auditory brainstem responses (EABRs) at experimental Days 7, 11, 17, 24, and 31. The animals were euthanized at Day 31, and the inner ears were dissected for immunohistochemistry investigation.


Implanted DRG cells, identified by enhanced green fluorescent protein fluorescence and a neuronal marker, were found close to Rosenthal canal in the adult inner ear for up to 4 weeks after transplantation. Extensive neurite projections clearly, greater than in nontreated animals, were observed to penetrate the bony modiolus and reach the spiral ganglion region in animals supplied with CES and/or NGF. There was, however, no significant difference in the thresholds of EABRs between DRG-transplanted animals supplied with CES and/or NGF and DRG-transplanted animals without CES or NGF supplement.


The results suggest that CES and/or NGF can stimulate neurite outgrowth from implanted neurons, although based on EABR measurement, these interventions did not induce functional connections to the central auditory pathway. Additional time or novel approaches may enhance functional responsiveness of implanted cells in the adult cochlea

Place, publisher, year, edition, pages
Lippincott Williams & Wilkins, 2009. Vol. 30, no 4, 551-558 p.
Keyword [en]
Chronic electrical stimulation; Dorsal root ganglion; Hair cell; Hearing loss; Nerve growth factor; Neurite; Spiral ganglion; Transplantation; Translational research
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-101018DOI: 10.1097/MAO.0b013e31819fe70aISI: 000276925800019PubMedID: 19395986OAI: diva2:665054
Available from: 2013-11-18 Created: 2013-11-18 Last updated: 2013-11-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Olivius, Petri
In the same journal
Otology and Neurotology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 31 hits
ReferencesLink to record
Permanent link

Direct link