liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Methods for integrating fluorimetry in the study of hearing organ structure and function
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden.
Huddinge Hospital, Sweden.
1997 (English)In: Hearing Research, ISSN 0378-5955, E-ISSN 1878-5891, Vol. 106, no 1-2, 29-38 p.Article in journal (Refereed) Published
Abstract [en]

The measurement of function in the intact organ of Corti has up to now been achieved by three methods: electrophysiology, mechanical measurement and biochemical analysis. The two former methods have supplied information at the level of single identified cells. We have used a fourth method, optical fluorimetry, to measure hair cell function at the cellular level in the intact organ of Corti. Here we describe the methods involved in fluorescence labelling and video-enhanced microscopy in combination with electrophysiological recording of cochlear microphonic (CM) and summating potentials (SP). The guinea pig temporal bone containing an intact ear drum, ossicular chain and cochlea can be maintained in the isolated state by perfusion of the scala tympani with oxygenated tissue culture medium. Substances added to the perfusate readily diffuse through the basilar membrane into the organ of Corti. In this way cells in the organ can be stained by a number of fluorescent probes which label different structures and functions. Here we have used two dyes which label mitochondria and fluoresce with an intensity proportional to metabolic activity. By simultaneous measurement of CM and SP the functional state of the organ can be monitored.

Place, publisher, year, edition, pages
Elsevier, 1997. Vol. 106, no 1-2, 29-38 p.
Keyword [en]
Auditory system; Hearing; Organ of Corti; Hair cell; Fluorescence microscopy; Cochlear potential
National Category
URN: urn:nbn:se:liu:diva-101082PubMedID: 9112105OAI: diva2:665127
Available from: 2013-11-19 Created: 2013-11-19 Last updated: 2013-11-28Bibliographically approved

Open Access in DiVA

No full text

Other links

PubMedLink to article

Search in DiVA

By author/editor
Fridberger, Anders
In the same journal
Hearing Research

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 188 hits
ReferencesLink to record
Permanent link

Direct link