Collagen Type I: A Promising Scaffold Material for Tissue Engineering and Regenerative Medicine
2012 (English)In: Type I collagen: biological functions, synthesis & medicinal applications / [ed] Maria Eduarda Henriques and Marcio Pinto, Nova Science Publishers, Inc., 2012, 1-43 p.Chapter in book (Other academic)
It is now recognized that biological macromolecules such as components of the extracellular matrix (ECM) are important as instructive templates in Regenerative Medicine applications. They are now increasingly used in the development of a new generation of bio-mimetic materials that allow for restoration of function when the self-renewal capacity of a tissue or organ cannot overcome degeneration caused by disease, injury or age-related wear. For example, macromolecules derived from connective tissue have been isolated, chemically modified, and used in medical applications ranging from tissue repair and reconstruction to drug and cell delivery systems. Common ECM macromolecules of vertebrates include collagen, proteoglycans, elastin, and other cell-interactive proteins such as fibronectin and laminin. Of these, type I collagen is the most abundant ECM macromolecule and is the primary scaffolding material that maintains the 3-dimensional structure of tissues and organs within the body. It also provides the micro-environmental milieu for cellular attachment, migration, and proliferation.
Animal-derived collagen is frequently used in tissue engineering applications due to its biocompatibility, but there are significant concerns about the immunogenicity of xenogeneic material as well as the possibility of pathogen transmission. Most recently, synthetic collagens and recombinant human collagens have been produced for medical application. Regardless of the source, however, macromolecules require processing and chemical treatment in order to improve their stability both in vitro and in vivo. This is most commonly achieved by cross-linking using a variety of agents. Cross-linking also allows for the development of “tailor-made” collagen-based biomaterials that possess specific properties for tissue engineering. Chemical cross-linkers such as glutaraldehyde and epoxy compounds are frequently used but their cytotoxicities have limited their clinical application. This has led to the use of zero-length cross-linkers such as carbodiimides and naturally derived agents such as genipin. Enzymatic cross-linking is becoming an attractive method to induce in situ biomaterial formation due to the mildness of the reaction. Naturally occurring enzymes such as transglutaminase are now commonly used. Photosensitizers used in combination with ultra-violet light irradiation can be used as exogenous cross-linkers. For example, riboflavin in combination with ultra-violet light is used clinically to augment the properties of collagen-based tissues such as the sclera and the cornea.
Collagen type I is a good candidate for tissue engineering and in vivo delivery systems for cells, proteins, and drugs. Important to its versatile and functional nature are its chemotactic properties, which promote cellular proliferation and differentiation, richness in cross-linking sites, and biodegradability. Collagen based delivery matrices have been reported to improve the results of cell delivery by improving cell viability.
Place, publisher, year, edition, pages
Nova Science Publishers, Inc., 2012. 1-43 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-101405ISBN: 978-1-62257-626-5OAI: oai:DiVA.org:liu-101405DiVA: diva2:666128
Table of Contents:
Collagen Type I: A Promising Scaffold Material for Tissue Engineering and Regenerative Medicine(Kimberly Merrett, Monika Kozak Ljunggren, Debasish Mondal, May Griffith, Mehrdad Rafat, Integrative Regenerative Medicine Centre, and Department of Clinical and Experimental Medicine, Linköping University, Linkoping, Sweden, and others)
Degradation of Type I Collagen and Pathogenesis of Infectious Diseases(Flávia Nader Motta, Clênia Azevedo, Carla Nunes de Araújo, Jaime M. Santana, Izabela M.D. Bastos, Laboratory of Pathogen-Host Interface, Department of Cell Biology, The University of Brasília, D.F., Brazil, and others)
Type I Collagen and its Utility as Specific Bone Marker(S.M. Friedman, S.N. Zeni, Oral and General Biochemistry Department, School of Dentistry, Buenos Aires University, Buenos Aires, Argentina, and others)
Development of 3-D Collagen Gel Vascularized Tissue-Engineered Constructs for Bone Replacement and Regeneration Using Embryonic and Postnatal Progenitors(Mani T. Valarmathi, John W. Fuseler, Department of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, South Carolina, USA)
Collagen I Synthesis, Biological Functions and Medical Applications during Systemic Inflammation(Ricardo Costa Petroni, Ester Correia Sarmento Rios, Francisco Garcia Soriano, Department of Emergency Medicine, University of São Paulo Medicine School, Brazil)
Collagen Scaffolds: Tissue Engineering and Repair(Eman Allam, Marco C. Bottino, Nouf Al-Shibani, L. Jack Windsor, Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, Indiana, and others)
Biomedical Implications of Type I Collagen: A Marine Perspective(Ramjee Pallela, Venkateswara Rao Janapala, Yoon-Bo Shim, Se-Kwon Kim, Institute of Biophysio Sensor Technology, Department of Chemistry, Pusan National University, Busan, South Korea, and others)
Role of Extracellular Matrix in Wound Repair Process(Simona Martinotti, Bruno Burlando, Elia Ranzato, Dipartimento di Scienze e Innovazione Tecnologica, DiSIT, Universityof Piemonte Orientale “Amedeo Avogadro”, Alessandria, Italy)