liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Single layer centrifugation of stallion spermatozoa improves sperm quality compared with sperm washing
Swedish University of Agriculture Science, Sweden .
Swedish University of Agriculture Science, Sweden .ORCID iD: 0000-0002-5194-2124
Swedish University of Agriculture Science, Sweden .
2010 (English)In: Reproductive Biomedicine Online, ISSN 1472-6483, Vol. 21, no 3, 429-436 p.Article in journal (Refereed) Published
Abstract [en]

This study compared the effect on semen quality of different handling methods used in the preparation of stallion semen doses for artificial insemination. The three methods were (i) extending the ejaculate to 30-50 x 10(6)/ml, (ii) single layer centrifugation (SLC) and (iii) sperm washing (centrifugation without a colloid). An additional treatment was to add seminal plasma (SP) in various proportions to some SLC preparations. The resulting samples were evaluated for sperm motility by computer assisted sperm analysis, membrane integrity using the Nucleocounter SP-100 and chromatin integrity by the sperm chromatin structure assay. SLC samples consistently had better sperm quality than the extended samples. Sperm washing did not confer any beneficial effect compared with the extended samples and these samples had significantly worse sperm quality than the SLC samples (motility, P less than 0.01; viability, P less than 0.001). There was no evidence to suggest that adding SP to the SLC samples could enhance sperm motility for more than a few hours. Longer term cold storage of spermatozoa in the presence of small concentrations of SP resulted in a reduction in total motility and progressive motility compared with SLC alone. High concentrations of SP were detrimental to sperm survival. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 21, no 3, 429-436 p.
Keyword [en]
chromatin integrity; seminal plasma; SLC; sperm motility; sperm washing; stallion
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-101727DOI: 10.1016/j.rbmo.2010.03.027ISI: 000282354100023OAI: diva2:666642
Available from: 2013-11-23 Created: 2013-11-22 Last updated: 2015-03-12

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
In the same journal
Reproductive Biomedicine Online
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 40 hits
ReferencesLink to record
Permanent link

Direct link