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Selection of Boar Spermatozoa Using Centrifugation on a Glycidoxypropyltrimethoxysilane-Coated Silica Colloid
Swedish University of Agriculture Science SLU, Sweden .
Swedish University of Agriculture Science SLU, Sweden University of Concepcion, Chile .
Swedish University of Agriculture Science SLU, Sweden .
Qual Genet, Sweden .
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2009 (English)In: Journal of reproduction and development, ISSN 0916-8818, E-ISSN 1348-4400, Vol. 55, no 5, 547-552 p.Article in journal (Refereed) Published
Abstract [en]

Use of sperm separation methods such as density gradient centrifugation for selecting the best spermatozoa for animal breeding is constrained by the problem of dealing with the large volumes of ejaculate produced by the males of some species, such as boars. The purpose Of this Study was to compare density gradient centrifugation (DGC) with centrifugation on a single layer of colloid (SLC) for the preparation of ejaculated boar spermatozoa using Androcoll (TM)-P. There was no difference between the two techniques in terms of sperm motility or duration of motility after selection, and sperm motility was retained for at least 24 h longer in the centrifuged sperm preparations than in controls (uncentrifuged aliquots). Sperm motility was significantly better (Pless than0.001) in the centrifuged sperm preparations (means +/- sd: SLC 79.6 +/- 8.1% and DGC 74.2 +/- 12.0%) than in the uncentrifuged controls (62.9 +/- 12.7%). The mean yield of motile spermatozoa for SLC was 67.5 +/- 25.6%, and for DGC it was 59.6% +/- 22.3%, (not significant, ns). Sperm survival was significantly increased by colloid centrifugation (control 3.1 +/- 0.3 days, SLC 5.5 +/- 0.79 days, DGC 5.75 +/- 0.62 days; Pless than0.001 for uncentrifuged versus centrifuged, SLC vs. DGC, ns). Moreover, boar spermatozoa could be stored for 24 h before centrifugation without: any detrimental effect on sperm motility or duration of motility. In a further experiment, larger volumes of ejaculate were processed easily on a modified SLC, indicating that this method may be practical for processing large volumes of boar ejaculates.

Place, publisher, year, edition, pages
Society for Reproduction and Development , 2009. Vol. 55, no 5, 547-552 p.
Keyword [en]
Boar spermatozoa; Colloids; Density gradient centrifugation; Single layer centrifugation
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-101737ISI: 000271357000015OAI: oai:DiVA.org:liu-101737DiVA: diva2:666653
Available from: 2013-11-23 Created: 2013-11-22 Last updated: 2017-12-06

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Rodriguez-Martinez, Heriberto

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