Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen
2009 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 114, no 1-3, 193-202 p.Article in journal (Refereed) Published
The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E (TM)). Sperm motility, sperm chromatin structure, membrane integrity and mitorchondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E (TM) significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI. (c) 2008 Elsevier B.V. All rights reserved.
Place, publisher, year, edition, pages
Elsevier Masson , 2009. Vol. 114, no 1-3, 193-202 p.
Frozen-thawed stallion semen; Colloid separation; CASA; Kinematics; Sperm morphology (ASMA); Membrane integrity; Mitochondrial function; SCSA; Sperm subpopulations
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-101740DOI: 10.1016/j.anireprosci.2008.08.025ISI: 000267144100021OAI: oai:DiVA.org:liu-101740DiVA: diva2:666656