liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effects of Straw Volume and Equex-STM (R) on Boar Sperm Quality after Cryopreservation
Chulalongkorn University, Thailand .
Chulalongkorn University, Thailand .
Chulalongkorn University, Thailand .
Swedish University of Agriculture Science SLU, Sweden .ORCID iD: 0000-0002-5194-2124
Show others and affiliations
2009 (English)In: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 44, no 1, 69-73 p.Article in journal (Refereed) Published
Abstract [en]

The present experiments were designed to study the effect of adding the detergent Equex-STM (R) to freezing extender, and of straw volume (0.25 ml vs 0.5 ml), on boar sperm quality after cryopreservation. Three ejaculates from each of four purebred boars (three Landrace and one Yorkshire) were collected and frozen with a lactose-egg yolk extender containing glycerol with or without 1.5% Equex-STM (R). The extended semen was loaded into either 0.25- or 0.5-ml straws. The straws were placed in liquid nitrogen (LN2) vapour approximately 3 cm above the level of LN2 for 20 min and then were plunged into LN2. Thawing was achieved in warm water at 50 degrees C for 12 s and then was incubated in a 38 degrees C water-bath for 30 min before evaluating sperm quality. Results showed that the individual motility, viability and acrosomal normal apical ridge (NAR) were improved (p less than 0.001) when Equex-STM (R) was added to the freezing extender. There was no difference (p = 0.48) in sperm motility between 0.25- and 0.5-ml straws when Equex-STM (R) was added. The percentages of viable and of NAR sperm in 0.5-ml straws were higher than those in 0.25-ml straws (p = 0.02, p = 0.0003 respectively). The percentages of membrane intact sperm evaluated using the short hypo-osmotic swelling test were not affected by straw volume or the adding of Equex-STM (R) (p greater than 0.05). The results of these investigations suggested that Equex-STM (R) exerts a beneficial effect on the quality of cryopreserved boar semen and this cryopreservation protocol was favourable for a 0.5-ml straw.

Place, publisher, year, edition, pages
Wiley-Blackwell , 2009. Vol. 44, no 1, 69-73 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-101750DOI: 10.1111/j.1439-0531.2007.00996.xISI: 000262487500012OAI: oai:DiVA.org:liu-101750DiVA: diva2:666666
Available from: 2013-11-23 Created: 2013-11-22 Last updated: 2017-12-06

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Rodriguez-Martinez, Heriberto

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
In the same journal
Reproduction in domestic animals
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 66 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf