Colloidal Centrifugation of Stallion Semen: Changes in Sperm Motility, Velocity, and Chromatin Integrity during Storage
2009 (English)In: Journal of Equine Veterinary Science, ISSN 0737-0806, E-ISSN 1542-7412, Vol. 29, no 1, 24-32 p.Article in journal (Refereed) Published
The current study investigated the changes in sperm quality (motility, velocity, and chromatin integrity) occurring during storage at room temperature or 5 degrees C for up to 48 hours in spermatozoa after extension or single-layer centrifugation (SLC) throught Androcoll-E. In unselected samples, all parameters of sperm quality deteriorated significantly during storage (P less than .01), although the deterioration was faster at room temperature (22-30 degrees C) than for cool storage (P less than .01). The SLC-selected spermatozoa had higher motility, velocity, and chromatin integrity than the overall unselected population (motility: selected 85 +/- 10%, unselected 56 +/- 13%; P less than .001; velocity: selected 85.1 +/- 13 mu m/second, unselected 63.5 +/- 15 mu m/second; P less than .001; and DFI selected 12.2 +/- 4.8 mu m/second; unselected 23.6 +/- 7.4 mu m/second; P less than .001). Furthermore, sperm quality did not deteriorate with storage in the SLC-selected samples, either at room temperature (22-30 degrees C for 24 hours) or cooled to 4 degrees C (for at least 48 hours), whereas a significant deterioration in sperm quality was observed in the unselected sperm samples (P less than .01). Thus, room temperature storage of SLC-selected spermatozoa may be an option for insemination doses from stallions whose spermatozoa do not tolerate cooling. In addition, a new sperm analyzer, the Qualisperm, showed good correlation with subjective motility assessment (r = 0.8, P less than .001), was user-friendly, and provided a reasonable volume of data. This instrument may be a useful adjunct to sperm quality assessment at the study.
Place, publisher, year, edition, pages
WB Saunders , 2009. Vol. 29, no 1, 24-32 p.
Single-layer centrifugation; Stallion spermatozoa; Qualisperm motility and velocity; Chromatin integrity
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-101755DOI: 10.1016/j.jevs.2008.11.006ISI: 000262620900010OAI: oai:DiVA.org:liu-101755DiVA: diva2:666670