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Morphological features of spermatozoa of swamp buffalo ai bulls, inThailand
Swedish University of Agriculture Science, Sweden.
Department of Livestock Development, Artificial Insemination Centre, Khon Kaen, Thailand.
Swedish University of Agriculture Science, Sweden .
Swedish University of Agriculture Science, Sweden.ORCID iD: 0000-0002-5194-2124
2007 (English)In: Journal of Veterinary Medicine A, ISSN 0931-184X, E-ISSN 1439-0442, Vol. 54, no 4, 169-178 p.Article in journal (Refereed) Published
Abstract [en]

The appearance and incidence of sperm abnormalities was studied in 115 ejaculates, collected periodically over I year covering all seasons from five mature, healthy swamp buffalo (Bubalus bubalis) bulls reared under tropical conditions and serving as the current source of semen for artificial insemination (Al) in Thailand. Light microscopy of stained smears was used to investigate sperm head shape morphology, while unstained wet smears were used to examine other sperm abnormalities. The most commonly found morphological aberrations were pear-shaped spermatozoa, knobbed acrosomes, proximal cytoplasmic droplets, simple bent tails and coiled tails under the head, whose ultrastructure (scanning electron microscopy) corresponded to what has been found in other species of bovidae, including varieties of buffalo. The mean prevalence (as least squares mean SEM) of sperm abnormalities was low (below 15%), corresponding to healthy spermiograms. The younger bulls (less than 10 years old, n = 3) had less abnormalities than the older ones (10.1 +/- 0.6% versus 14.1 +/- 0.8%, P less than 0.001, n = 2), including abnormalities of sperm head shape (1.1 +/- 0.3% versus 3.6 +/- 0.3, P less than 0.001), acrosome defects with knobbed acrosomes (1.1 +/- 0.2% versus 1.2 +/- 0.3%, P less than 0.001), spermatozoa with proximal cytoplasmic droplets (2.7 +/- 0.1 % versus 1.4 +/- 0.2%, P less than 0.00 1), defective mid-pieces (0.2 +/- 0.1% versus 0.3 +/- 0.1%) and abnormal sperm tails (3.1 +/- 0.3% versus 5.7 +/- 0.4%, P less than 0.001). The within-bull effect of the year solely affected the incidence of pear-shaped spermatozoa while the incidences of abnormal contour, variable size of sperm head shapes, abnormal mid-piece and simple bent tail among bulls were affected by ejaculate (week of collection). Interaction between age and ejaculate affected only the prevalence of spermatozoa with proximal cytoplasmic droplets. In conclusion, the types of defects encountered were similar to those found in other bovidae, with a very low prevalence over the year the Al sires were followed through.

Place, publisher, year, edition, pages
Wiley-Blackwell , 2007. Vol. 54, no 4, 169-178 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-101780DOI: 10.1111/j.1439-0442.2007.00895.xISI: 000246370700001OAI: oai:DiVA.org:liu-101780DiVA: diva2:666695
Available from: 2013-11-23 Created: 2013-11-22 Last updated: 2017-12-06

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Rodriguez-Martinez, Heriberto

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