Detection of early changes in sperm membrane integrity pre-freezing can estimate post-thaw quality of boar spermatozoa
2007 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 97, no 1-2, 74-83 p.Article in journal (Refereed) Published
A recently developed triple staining (SNARF-1/YO-PRO-1/ethidium homodimer) was used to assess early changes in boar sperm membrane integrity (MI) with the results of cryopreservation procedures and to seek for correlations among MI-spermatozoa in pre-freeze semen and its freezeability. Ejaculates from five boars were evaluated in the fresh and frozen-thawed (FT) state, and its freezeability defined as % of membrane intactness, MI% (MI% = % of FT-spermatozoa with intact membranes x 100 divided by the % of prefreeze spermatozoa with intact membranes) estimated. Significant differences were found among boars for freezeability (MI%) and motility post-thaw (%). Interestingly, significant correlations were found between the percentage of YO-PRO-1-positive spermatozoa and freezeability (R = 0.440, p less than 0.01), indicating this new triple staining can be used to safely disclose among ejaculates prior to freezing. (c) 2006 Elsevier B.V. All rights reserved.
Place, publisher, year, edition, pages
Elsevier Masson , 2007. Vol. 97, no 1-2, 74-83 p.
boar; cryopreservation; plasmalemma; YO-PRO-1; SNARF-1 ethidium homodimer; freezability
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-101786DOI: 10.1016/j.anireprosci.2005.12.014ISI: 000242543200008OAI: oai:DiVA.org:liu-101786DiVA: diva2:666791