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Assessment of semen quality in Swamp Buffalo AI Bulls in Thailand
Kasetsart University, Thailand .
Swedish University of Agriculture Science, Sweden .ORCID iD: 0000-0002-5194-2124
2007 (English)In: Italian Journal of Animal Science, ISSN 1594-4077, E-ISSN 1828-051X, Vol. 6, 701-704 p.Article in journal (Refereed) Published
Abstract [en]

Characteristic of Thai swamp buffalo bulls semen used for artificial insemination (AI) in Thailand, aspects relevance in freezing and thawing of semen are review. Semen and sperm characteristics were evaluated included sperm count, motility (assessed subjectively and by CASA), morphology (using phase-contrast light microscopy and SEM), plasma membrane integrity (PMI) (using a hypo-osmotic swelling test [HOST]) and SYBR-14/propidium iodide [PI]), plasma membrane stability (PMS) (using Annexin-V/PI) and deoxyribonucleic acid (DNA) integrity (using SCSA and flow cytometry [FCM]). The average ejaculate volume was about 3.0-4.0 mL, with good viability (PMI measured by the HOST) and motility (greater than65% and greater than70%, respectively). Sperm concentration ranged from 1.1 to 1.2 billion/mL, being also affected by bull age. Whereas semen quality (including sperm output, pH and initial sperm motility) did not differ between the seasons. Few spermatozoa (less than15%/ejaculate) had abnormal morphology with abnormalities resembling those in other bovidae. In FT semen, PMI (using SYBR-14/PI) and PMS were highest in winter. Across seasons, similar to 50% of post-thaw spermatozoa depicted linear motility, a proportion that decreased to similar to 35% during incubation (38 degrees C for 60 minutes), without marking any seasonal difference. The sperm DNA was hardly damaged (with less than3% fragmentation, expressed as DNA fragmentation index [DFI]), among seasons.

Place, publisher, year, edition, pages
PAGEpress / PAGEPress Publications , 2007. Vol. 6, 701-704 p.
Keyword [en]
Swamp buffalo; Ejaculate volume; Sperm motility; Sperm morphology
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-101788ISI: 000207598400149OAI: diva2:666793
Available from: 2013-11-24 Created: 2013-11-22 Last updated: 2015-03-12

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Rodriguez-Martinez, Heriberto
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