liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Effects of exposure of pre-pubertal boars to di(2-ethylhexyl) phthalate on their frozen-thawed sperm viability post-puberty
Swedish University of Agriculture Science, Sweden; .
Swedish University of Agriculture Science, Sweden; .
Swedish University of Agriculture Science, Sweden; .
Swedish University of Agriculture Science, Sweden; .
Show others and affiliations
2006 (English)In: Andrologia, ISSN 0303-4569, E-ISSN 1439-0272, Vol. 38, no 5, 186-194 p.Article in journal (Refereed) Published
Abstract [en]

Late effects of pre-pubertal oral exposure to di(2-ethylhexyl) phthalate (DEHP), a plastic softener used in, for example, polyvinyl chloride-products, on semen quality in young boars have not been clear-cut. The aim of this study was to determine whether stress imposed on spermatozoa would reveal such effects. Semen was collected from post-pubertal boars (8-9 months of age), which had been exposed to 300 mg kg(-1) body weight of DEHP per os three times a week from 3 to 7 weeks of age and from control siblings given placebo (water). The semen was cryopreserved and examined for plasma membrane integrity post-thaw using the short hypo-osmotic swelling test and flow cytometry (propidium iodide /SYBR-14). Sperm motility was assessed by computer-assisted sperm analysis. No significant difference in plasma membrane integrity could be found between the groups. The DEHP-exposed group had a significantly lower percentage of linearly motile spermatozoa at 30 min (P less than 0.05) and 120 min (P less than 0.001) after thawing, and a larger amplitude of lateral displacement of the head 120 min after thawing (P less than 0.05), compared with controls. In summary, spermatozoa from boars pre-pubertally exposed to low doses of DEHP, showed kinematic deviations post-thaw that could be related to DEHP exposure.

Place, publisher, year, edition, pages
Wiley-Blackwell , 2006. Vol. 38, no 5, 186-194 p.
Keyword [en]
boar; di(2-ethylhexyl) phthalate; frozen-thawed; sperm motility; sperm viability
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-101793DOI: 10.1111/j.1439-0272.2006.00737.xISI: 000240401700005OAI: diva2:666798
Available from: 2013-11-24 Created: 2013-11-22 Last updated: 2015-03-12

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
In the same journal
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 43 hits
ReferencesLink to record
Permanent link

Direct link