Identification of sperm morphometric subpopulations in two different portions of the boar ejaculate and its relation to postthaw quality
2005 (English)In: Journal of Andrology, ISSN 0196-3635, Vol. 26, no 6, 716-723 p.Article in journal (Refereed) Published
A statistical approach using sequentially principal component analysis (PCA), clustering, and discriminant analyses was. developed to identify sperm morphometric subpopulations in well-defined portions of the fresh boar ejaculate. Semen was obtained as 2 portions (the first 10 mL of the sperm-rich fraction and the rest of. the ejaculate, respectively) and frozen using a conventional protocol. Before freezing, an aliquot was Used for computer-assisted. sperm morphometry analysis (ASMA). Postthaw quality was evaluated using computer-assisted sperm analysis (CASA), and an annexin-V/PI assay evaluated sperm membranes. The PCA revealed that 3 variables represented more than 78% of the cumulative variance in sperm subpopulations. The clustering and discriminant analyses, based on 5780 individual spermatozoa, revealed the existence of 4 sperm subpopulations. The relative percentage of these subpopulations Varied between boar and ejaculate portions. Linear regression models based on measured morphometric characteristics could account for up to 36% of the percentage of intact sperm membranes postthaw. The ASMA protocol used in our study was useful to detect subtle morphometric differences between spermatozoa, and the combination of this analysis with a multivariate statistical procedure gave hew information on the biological characteristics of boar ejaculates that is not given by conventional sperm analysis.
Place, publisher, year, edition, pages
American Society of Andrology , 2005. Vol. 26, no 6, 716-723 p.
ASMA; sperm subpopulations; cryopreservation; cluster analysis
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-101809DOI: 10.2164/jandrol.05030ISI: 000232605400011OAI: oai:DiVA.org:liu-101809DiVA: diva2:666806