Effect of storage in short- and long-term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa
2006 (English)In: International Journal of Andrology, ISSN 0105-6263, E-ISSN 1365-2605, Vol. 29, no 5, 543-552 p.Article in journal (Refereed) Published
For artificial insemination (AI) in pigs, preservation of liquid boar semen at 16-20 degrees C is still common practice as sperm cryopreservation remains suboptimal in this species. To meet the different needs of the swine industry, several extenders have been developed to preserve semen in liquid form for short- and long-term storage. In the present study, three different commercial extenders devised for short-term (BTS+) or long-term preservation (MR-A and X-Cell), were used to test whether storage of semen from four mature, fertile boars at 17 degrees C for 96 h would affect sperm characteristics relevant for fertility, such as motility, membrane integrity and chromatin stability. Computer-assisted sperm analysis, and stainings with the acylated membrane dye SYBR-14/propidium iodide, and acridine orange in connection with flow cytometry were used to evaluate these variables. Percentages of total motile spermatozoa decreased slightly, but significantly, after 72-96 h. While membrane integrity values varied during the period of study, no significant changes in either membrane integrity or chromatin stability were, however, registered. This suggests a customary 96-day storage at 17 degrees C in these extenders was too short an interval to cause losses of integrity in nuclear DNA in the boar population studied.
Place, publisher, year, edition, pages
Wiley-Blackwell , 2006. Vol. 29, no 5, 543-552 p.
boar; chromatin stability; liquid semen; sperm viability; storage
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-101795DOI: 10.1111/j.1365-2605.2006.00694.xISI: 000240318600007OAI: oai:DiVA.org:liu-101795DiVA: diva2:666821