liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Estimation of removed uremic toxin indoxyl sulphate during hemodialysis by using optical data of the spent dialysate
Tallinn University of Technology, Estonia .
Östergötlands Läns Landsting, Heart and Medicine Center, Department of Nephrology. Linköping University, Department of Medical and Health Sciences, Internal Medicine. Linköping University, Faculty of Health Sciences.
Tallinn University of Technology, Estonia.
Tallinn University of Technology, Estonia.
Show others and affiliations
2013 (English)In: IEEE Engineering in Medicine and Biology Society. Conference Proceedings, ISSN 1557-170X, 6707-6710 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to explore the possibility to determine the amount of total removed Indoxyl Sulphate (TR_IS) during dialysis session, an optical method utilizing absorbance and fluorescence spectral data of the spent dialysate was used. Eight uremic patients from Linköping, Sweden and 10 from Tallinn, Estonia, were studied during dialysis treatments. Dialysate samples were taken during each treatment and analyzed at a laboratory. Fluorescence and absorbance spectra of the spent dialysate were measured with spectrofluorophotometer and spectrophotometer. The spectral values were transformed into IS concentration using multiple linear regression model from the total material noted as optical method (Opt). IS concentration was estimated using high-performance liquid chromatography (HPLC) method as a reference. TR_IS values were calculated. Achieved results were compared regarding mean values and SD and collated with the amount of total removed urea value (TR_Urea) for the same dialysis procedures. Mean TR value ± SD (mg) for urea was 28 947 ± 9 241; TR for IS was 151.4 ± 87.3 estimated by HPLC and 149.4 ± 84.9 estimated by Opt. The TR_IS values were not significantly different (p ≤ 0.05). This study indicates, that it is possible to estimate TR_IS using only spectral values of the spent dialysate and the parameter can be used for quantifying the elimination of protein bound uremic toxins during the dialysis procedure.

Place, publisher, year, edition, pages
IEEE , 2013. 6707-6710 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-102328DOI: 10.1109/EMBC.2013.6611095PubMedID: 24111282OAI: diva2:676400
35th Annual International Conference of Engineering in Medicine and Biology Society (EMBC), 3-7 July 2013, Osaka, Japan
Available from: 2013-12-05 Created: 2013-12-05 Last updated: 2014-08-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Uhlin, FredrikFridolin, Ivo
By organisation
Department of NephrologyInternal MedicineFaculty of Health Sciences
In the same journal
IEEE Engineering in Medicine and Biology Society. Conference Proceedings
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 169 hits
ReferencesLink to record
Permanent link

Direct link