liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Spectral correlation analysis of Amyloid beta plaque inhomogeneity from double staining experiments
Norwegian University of Science and Technology, Norway .
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Norwegian University of Science and Technology, Norway .
Norwegian University of Science and Technology, Norway .
2013 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 18, no 10Article in journal (Refereed) Published
Abstract [en]

A spectral correlation algorithm for the analysis of hyperspectral fluorescence images is proposed by Ellingsen et al. [J. Biomed. Opt. 18, 020501 (2013)]. Here, it is applied to the analysis of double-stained A beta amyloid plaques being related to the Alzheimers disease (AD). Sections of APP/PS1 AD mice model brains are double stained with luminescent-conjugated oligothiophenes, known to bind to amyloid protein deposits. Hyperspectral fluorescence images of the brain sections are recorded and by applying the correlation algorithm the spectral inhomogeneity of the double-stained samples is mapped in terms of radial distribution and spectral content. To further investigate the progression of A beta amyloid plaque formation, 19 AD mice of different ages up to 23 months are characterized, enabling a statistical analysis of the plaque heterogeneity. In accordance with recent findings by Nystrom et al. [ACS Chem. Biol. 8, 1128-1133 (2013)], the spectral distribution within A beta plaques is found to vary with age throughout the lifespan of the mouse. With the new correlation algorithm, it is possible to quantify the spectral abundance of the two stains depending on the relative distance from the plaque center and mouse age. Thus, we demonstrate the use of the correlation analysis approach in double-staining experiments and how it is possible to relate these to structural/spectral changes in biological samples. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Place, publisher, year, edition, pages
Society of Photo-optical Instrumentation Engineers (SPIE) , 2013. Vol. 18, no 10
Keyword [en]
double stain, hyperspectral imaging, spectral analysis, Alzheimers disease, amyloid plaque
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-102404DOI: 10.1117/1.JBO.18.10.101313ISI: 000326563000019OAI: diva2:677361

Funding Agencies|EU-FP7 Health Programme Project LUPAS||Swedish Alzheimer Foundation||

Available from: 2013-12-09 Created: 2013-12-09 Last updated: 2013-12-09

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Nyström, Sofie
By organisation
ChemistryThe Institute of Technology
In the same journal
Journal of Biomedical Optics
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 43 hits
ReferencesLink to record
Permanent link

Direct link