Comparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study
2013 (English)In: European Journal of Applied Physiology, ISSN 1439-6319, E-ISSN 1439-6327, Vol. 113, no 12, 2977-2989 p.Article in journal (Refereed) Published
The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a comprehensive explorative screening of the cellular processes in the muscle. less thanbrgreater than less thanbrgreater thanMicrodialysate samples were obtained from the shoulder muscle of healthy and myalgic subjects that performed a work and stress test. Samples from the baseline period and from the recovery period were analysed using gas chromatography-mass spectrometry (GC-MS) together with multivariate analysis to detect differences in extracellular content of metabolites between groups. Systematic differences in metabolites between groups were identified using multivariate analysis and orthogonal partial least square discriminate analysis (OPLS-DA). A complementary Mann-Whitney U test of group difference in individual metabolites was also performed. less thanbrgreater than less thanbrgreater thanA large number of metabolites were detected and identified in this screening study. At baseline, no systematic differences between groups were observed according to the OPLS-DA. However, two metabolites, l-leucine and pyroglutamic acid, were significantly more abundant in the myalgic muscle compared to the healthy muscle. In the recovery period, systematic difference in metabolites between the groups was observed according to the OPLS-DA. The groups differed in amino acids, fatty acids and carbohydrates. Myristic acid and putrescine were significantly more abundant and beta-d-glucopyranose was significantly less abundant in the myalgic muscle. less thanbrgreater than less thanbrgreater thanThis study provides important information regarding the metabolite content, thereby presenting new clues regarding the pathophysiology of the myalgic muscle.
Place, publisher, year, edition, pages
Springer Verlag (Germany) , 2013. Vol. 113, no 12, 2977-2989 p.
Metabolomics, Trapezius myalgia, Microdialysis, Repetitive work, Recovery, GC-MS, Metabolites
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-102492DOI: 10.1007/s00421-013-2716-6ISI: 000327087000009OAI: oai:DiVA.org:liu-102492DiVA: diva2:679193
Funding Agencies|Swedish Research Council|K2011-69X-21874-01-6|Swedish Council for Working life and Social research|2009-17612010-0913|2013-12-132013-12-122014-12-05