A high-performance glucose biosensor using covalently immobilised glucose oxidase on a poly(2,6-diaminopyridine)/carbon nanotube electrode
2013 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 116, 801-808 p.Article in journal (Refereed) Published
A highly-sensitive glucose biosensor amenable to ultra-miniaturisation was fabricated by immobilisation of glucose oxidase (GOx), onto a poly(2,6-diaminopyridine)/multi-walled carbon nanotube/glassy carbon electrode (poly(2,6-DP)/MWNT/GCE). Cyclic voltammetry was used for both the electrochemical synthesis of poly-(2,6-DP) on the surface of a MWNT-modified GC electrode, and characterisation of the polymers deposited on the GC electrode. The synergistic effect of the high active surface area of both the conducting polymer, i.e., poly-(2,6-DP) and MWNT gave rise to a remarkable improvement in the electrocatalytic properties of the biosensor. The transfer coefficient (alpha), heterogeneous electron transfer rate constant and Michaelis-Menten constant were calculated to be 0.6, 4 s(-1) and 0.20 mM at pH 7.4, respectively. The GOx/poly(2,6-DP)/MWNT/GC bioelectrode exhibited two linear responses to glucose in the concentration ranging from 0.42 mu M to 8.0 mM with a correlation coefficient of 0.95, sensitivity of 52.0 mu AmM-1 cm(-2), repeatability of 1.6% and long-term stability, which could make it a promising bioelectrode for precise detection of glucose in the biological samples. (C) 2013 Elsevier B.V. All rights reserved.
Place, publisher, year, edition, pages
Elsevier , 2013. Vol. 116, 801-808 p.
Glucose biosensor, Direct electron transfer, Glucose oxidase, MWNT/poly-(2, 6-diaminopyridine)
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-102852DOI: 10.1016/j.talanta.2013.07.068ISI: 000328176000114OAI: oai:DiVA.org:liu-102852DiVA: diva2:683823
Funding Agencies|European Commission|PIIF-GA-2009-254955|LIST - Linkoping University||Zanjan University Research Council||2014-01-072014-01-022015-05-27