liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Protective Induction of Hsp70 in Heat-Stressed Primary Myoblasts: Involvement of MAPKs
State Research Institute Centre Innovat Med, Lithuania .
Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Health Sciences.
State Research Institute Centre Innovat Med, Lithuania .
2013 (English)In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 114, no 9, 2024-2031 p.Article in journal (Refereed) Published
Abstract [en]

The involvement of extracellular signal-regulated kinases 1 and 2 (ERK1,2), stress kinase p38 and c-Jun NH2-terminal kinases 1 and 2 (JNK1,2) on Hsp70-upregulation following mild heat shock, and resulting cell protection, was studied on rabbit primary myoblasts. Cells subjected to heat stress (42 degrees C; 60min) showed a significantly enhanced amount of heat-shock-induced protein 70 (Hsp70), correlating with sustained phosphorylation of MAP kinases ERK1,2, inhibition of p38 and JNK1,2 activation. Induced Hsp70 did not autocrinally suppress activation of transcription factor c-Jun, suggesting involvement of the latter in the protection of myoblasts following heat shock. The inhibition of stress kinases p38, JNK1,2, and MEK1,2 by SP600125, SB203580, and UO126, respectively, established the involvement of JNK1,2 and p38 as upstream, and ERK1,2 as downstream targets of Hsp70 induction. Moreover, the effect of the MEK1,2 inhibitor UO126 revealed a new pathway of c-Jun activation by ERK1,2 in myogenic heat-stressed stem cells. The presented data show that transient activation of JNK1, JNK2, and p38 is necessary for Hsp70 induction and ensuing cell protection. In conclusion, affecting myogenic stem cell protective mechanisms might be a useful strategy in improving stem cell survival and their expanded application in therapy.

Place, publisher, year, edition, pages
Wiley-Blackwell , 2013. Vol. 114, no 9, 2024-2031 p.
Keyword [en]
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-102789DOI: 10.1002/jcb.24550ISI: 000327699500009OAI: diva2:683859

Funding Agencies|Lithuanian State Science and Studies Foundation|T-65/07U-04001C-07023|

Available from: 2014-01-07 Created: 2013-12-26 Last updated: 2014-01-07

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Brunk, Ulf
By organisation
Division of Drug ResearchFaculty of Health Sciences
In the same journal
Journal of Cellular Biochemistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 69 hits
ReferencesLink to record
Permanent link

Direct link