Low density DNA arrays are of growing interest in the field of fast clinical/environmental analysis; for example in clinical analysis these can find application in areas as early diagnosis of genetic diseases or as support in the diagnosis of genetically associated diseases.
Coeliac disease, a small intestinal inflammation triggered by the intake of gluten, has been shown to affect only genetically predisposed individuals. Relation between Coeliac disease and two Human Leukocyte Antigens (HLA) genes, DQ2 and DQ8, has been reported, with almost 100% of the affected patients carrying at least one of them.
In this seminar the development of a low density electrochemical genosensor array for medium resolution typing of Coeliac disease associated HLA genes, is presented.
The proposed array was based on an enzymatic sandwich assay format performed at a photolitographically fabricated electrode array.
The optimisation of different aspects as surface chemistry, assay conditions, probe's design and single stranded DNA generation, together with real sample analysis will be present.
2013. 21- p.
COST Thematic Workshop: "Biomimetic structures and DNA technology in biosensing", 8-10 April 2013, Bratislava