A universal method for species identification of mammals utilizing next generation sequencing for the analysis of DNA mixtures
2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 12, e83761- p.Article in journal (Refereed) Published
Species identification can be interesting in a wide range of areas, for example, in forensic applications, food monitoring and in archeology. The vast majority of existing DNA typing methods developed for species determination, mainly focuses on a single species source. There are, however, many instances where all species from mixed sources need to be determined, even when the species in minority constitutes less than 1 % of the sample. The introduction of next generation sequencing opens new possibilities for such challenging samples. In this study we present a universal deep sequencing method using 454 GS Junior sequencing of a target on the mitochondrial gene 16S rRNA. The method was designed through phylogenetic analyses of DNA reference sequences from more than 300 mammal species. Experiments were performed on artificial species-species mixture samples in order to verify the method's robustness and its ability to detect all species within a mixture. The method was also tested on samples from authentic forensic casework. The results showed to be promising, discriminating over 99.9 % of mammal species and the ability to detect multiple donors within a mixture and also to detect minor components as low as 1 % of a mixed sample.
Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 12, e83761- p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-103045DOI: 10.1371/journal.pone.0083761ISI: 000328735700137PubMedID: 24358309OAI: oai:DiVA.org:liu-103045DiVA: diva2:686216