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Comparison of a capillary gel electrophoresis-based multiplex PCR assay and ribosomal intergenic transcribed spacer-2 amplicon sequencing for identification of clinically important Candida species
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Microbiology.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Microbiology.
2014 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 96, 81-83 p.Article in journal (Refereed) Published
Abstract [en]

The performance of a commercially available Seegene Seeplex STI Master Panel 3 multiplex PCR for Candida species identification was compared with an internal transcribed spacer 2 (ITS2) PCR assay. We found that the Seeplex assay was specific for identification of C. albicans, C. krusei, C. parapsilosis, C. glabrata, C. tropicalis and C. dubliniensis.

Place, publisher, year, edition, pages
2014. Vol. 96, 81-83 p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-102441DOI: 10.1016/j.mimet.2013.11.004ISI: 000329771000015PubMedID: 24252582OAI: oai:DiVA.org:liu-102441DiVA: diva2:686225
Available from: 2014-01-10 Created: 2013-12-10 Last updated: 2017-12-06

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Monstein, Hans-JürgTärnberg, MariaJohansson, Anders G

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