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High Concentration but Low Activity of Hepatocyte Growth Factor in Periodontitis
Univ Orebro, Sch Hlth & Med Sci, Div Clin Med, Orebro, Sweden; PEAS Institute, Linköping and Örebro University, Sweden.
Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Public Dental Health Care.
PEAS Institute, Linköping, Sweden.
Örebro University, Sweden.
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2014 (English)In: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 85, no 1, 113-122 p.Article in journal (Refereed) Published
Abstract [en]

Background: High levels of hepatocyte growth factor (HGF), a healing factor with regenerative and cytoprotective effects, are associated with inflammatory diseases, including periodontitis. HGF biologic activity requires binding to its receptors, the proto-oncogene c-Met and heparan sulfate proteoglycan (HSPG). This study investigates HGF expression and its relationship to subgingival microbiota in medically healthy individuals with and without periodontitis.

Methods: Saliva, gingival crevicular fluid (GCF), and blood samples from 30 patients with severe periodontitis and 30 healthy controls were analyzed for HGF concentration using enzyme-linked immunosorbent assay and binding affinity for HSPG and c-Met using surface plasmon resonance. The regenerative effects of saliva from three patients and controls were analyzed in an in vitro model of cell injury. Subgingival plaques were analyzed for the presence of 18 bacterial species.

Results: Patients with periodontitis showed higher HGF concentrations in saliva, GCF, and serum (P <0.001); however, the binding affinities for HSPG and c-Met were reduced in GCF and saliva (P <0.002). In contrast to the controls, saliva from patients showed no significant regenerative effect over time on gingival epithelial cells. Compared with controls, patients had a higher prevalence of periodontally related bacteria.

Conclusions: Higher circulatory HGF levels indicate a systemic effect of periodontitis. However, the HGF biologic activity at local inflammation sites was reduced, and this effect was associated with the amount of periodontal bacteria. Loss of function of healing factors may be an important mechanism in degenerative processes in periodontally susceptible individuals.

Place, publisher, year, edition, pages
American Academy of Periodontology , 2014. Vol. 85, no 1, 113-122 p.
Keyword [en]
Gingival crevicular fluid, hepatocyte growth factor, microbiology, periodontal diseases, saliva, serum
National Category
Clinical Medicine Dentistry Cell and Molecular Biology
URN: urn:nbn:se:liu:diva-103611DOI: 10.1902/jop.2013.130003ISI: 000331139400016OAI: diva2:689564
Available from: 2014-01-21 Created: 2014-01-21 Last updated: 2015-06-11Bibliographically approved

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Lönn, JohannaStarkhammar Johansson, CarinNayeri, FaribaRavald, Nils
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Division of Cardiovascular MedicineFaculty of Health SciencesPublic Dental Health CareDivision of Microbiology and Molecular MedicineDepartment of Infectious Diseases
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Journal of Periodontology
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