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Microtrauma stimulates rat Achilles tendon healing via an early gene expression pattern similar to mechanical loading
Linköping University, Department of Clinical and Experimental Medicine, Division of Inflammation Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Inflammation Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Orthopaedics in Linköping.
Linköping University, Department of Clinical and Experimental Medicine, Division of Inflammation Medicine. Linköping University, Faculty of Health Sciences.
2014 (English)In: Journal of applied physiology, ISSN 8750-7587, E-ISSN 1522-1601, Vol. 116, no 1, 54-60 p.Article in journal (Refereed) Published
Abstract [en]

Mechanical loading increases the strength of healing tendons, but also induces small localized bleedings. Therefore, it is unclear if increased strength after loading is a response to mechanotransduction or microtrauma. We have previously found only five genes to be up-regulated 15 min after a single loading episode, of them four were transcription factors. These genes are followed by hundreds of genes after 3 h, many of them involved in inflammation. We now compared healing in mechanically unloaded tendons with or without added microtrauma induced by needling of the healing tissue. Nineteen rats received Botox into the calf muscle to reduce loading, and the Achilles tendon was transected. Ten rats were randomized to needling days 2-5. Mechanical testing on day 8 showed increased strength by 45% in the needling group. Next, another 24 rats were similarly unloaded, and 16 randomized to needling on day 5 after transection. Nineteen characteristic genes, known to be regulated by loading in this model, were analyzed by qRT-PCR. Four of these genes were regulated 15 min after needling. Three of them (Egr1, c-Fos, Rgs1) were among the five regulated genes after loading in a previous study. Sixteen of the 19 genes were regulated after 3 h, in the same way as after loading. In conclusion, needling increased strength, and there was a striking similarity between the gene expression response to needling and mechanical loading. This suggests that the response to loading in early tendon healing can, at least in part, be a response to microtrauma.

Place, publisher, year, edition, pages
American Physiological Society , 2014. Vol. 116, no 1, 54-60 p.
Keyword [en]
qRT-PCR; unloading; mechanical testing; early response genes; inflammation
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-103870DOI: 10.1152/japplphysiol.00741.2013ISI: 000329196100008OAI: oai:DiVA.org:liu-103870DiVA: diva2:692536
Available from: 2014-01-31 Created: 2014-01-30 Last updated: 2017-12-06

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Hammerman, MalinAspenberg, PerEliasson, Pernilla

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Division of Inflammation MedicineFaculty of Health SciencesDepartment of Orthopaedics in Linköping
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