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Inhibition of sodium-linked glucose reabsorption normalizes diabetes-induced glomerular hyperfiltration in conscious adenosine A(1)-receptor deficient mice
Uppsala University, Sweden .
Uppsala University, Sweden .
Karolinska Institute, Sweden .
Uppsala University, Sweden .
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2014 (English)In: Acta Physiologica, ISSN 1748-1708, E-ISSN 1748-1716, Vol. 210, no 2, 440-445 p.Article in journal (Refereed) Published
Abstract [en]

AimGlomerular hyperfiltration is commonly observed in diabetics early after the onset of the disease and predicts the progression of nephropathy. Sustained hyperglycaemia is also closely associated with kidney hypertrophy and increased electrolyte and glucose reabsorption in the proximal tubule. In this study, we investigated the role of the increased tubular sodium/glucose cotransport for diabetes-induced glomerular hyperfiltration. To eliminate any potential confounding effect of the tubuloglomerular feedback (TGF) mechanism, we used adenosine A(1)-receptor deficient (A(1)AR(-/-)) mice known to lack a functional TGF mechanism and compared the results to corresponding wild-type animals (A(1)AR(+/+)). MethodsDiabetes was induced by an intravenous bolus injection of alloxan. Glomerular filtration rate (GFR) was determined in conscious mice by a single bolus injection of inulin. The sodium/glucose cotransporters were inhibited by phlorizin 30min prior to GFR measurements. ResultsNormoglycaemic animals had a similar GFR independent of genotype (A(1)AR(+/+) 23311 vs. A(1)AR(-/-) 241 +/- 25Lmin(-1)), and induction of diabetes resulted in glomerular hyperfiltration in both groups (A(1)AR(+/+) 380 +/- 25 vs. A(1)AR(-/-) 336 +/- 35Lmin(-1); both Pless than0.05). Phlorizin had no effect on GFR in normoglycaemic mice, whereas it reduced GFR in both genotypes during diabetes (A(1)AR(+/+) 365 +/- 18 to 295 +/- 19, A(1)AR(-/-) 354 +/- 38 to 199 +/- 15Lmin(-1); both Pless than0.05). Notably, the reduction was more pronounced in the A(1)AR(-/-) (Pless than0.05). ConclusionThis study demonstrates that increased tubular sodium/glucose reabsorption is important for diabetes-induced hyperfiltration, and that the TGF mechanism is not involved in these alterations, but rather functions to reduce any deviations from a new set-point.

Place, publisher, year, edition, pages
Wiley , 2014. Vol. 210, no 2, 440-445 p.
Keyword [en]
adenosine; diabetes mellitus; glomerular hyperfiltration; tubuloglomerular feedback
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-104111DOI: 10.1111/apha.12152ISI: 000329519500020OAI: diva2:694699
Available from: 2014-02-07 Created: 2014-02-07 Last updated: 2014-02-07

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Palm, Fredrik
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Division of Drug ResearchFaculty of Health Sciences
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