Cilia-mediated Hedgehog signaling in Drosophila
2014 (English)Manuscript (preprint) (Other academic)
Cilia mediate vertebrate Hedgehog (Hh) signaling, and Hh deregulation results in several clinical manifestations, such as various cancers, obesity, cognitive disabilities and developmental malformations. Drosophila, cells are non-ciliated during development, which has led to the assumption that cilia mediated Hh signaling is restricted to vertebrates. Here, we identify and characterize a cilia mediated Hh pathway in Drosophila. We demonstrate that several fundamental key aspects of the vertebrate cilia pathway, such as ciliary localization of Smoothened and requirement of the intraflagellar transport system, are required in vivo and conserved from Drosophila to vertebrates. We show that Cos2 and Fused are required for the ciliary transport of Smoothened. Last, we show that olfaction in Drosophila requires cilia mediated Hh signaling in the olfactory sensory neurons. Taken together, our data clearly show that Hh signaling in Drosophila can be mediated by two pathways and that the cilia pathway is conserved from Drosophila to vertebrates.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-104703OAI: oai:DiVA.org:liu-104703DiVA: diva2:698616