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Increased lysosomal membrane permeabilization in oxidant-exposed macrophages of human fibrotic lungs
Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Respiratory Medicine.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences.
2013 (English)In: Journal of Cell Death, ISSN 1179-0660, Vol. 6, no 1, 69-74 p.Article in journal (Refereed) Published
Abstract [en]

A disrupted balance of reduced glutathione (GSH) and iron (Fe) and subsequent enhanced susceptibility of lysosomes of lung macrophages (LMs) to oxidants may play a role in lung fibrogenesis. We assessed cellular Fe/GSH, lysosomal membrane permeabilization (LMP), and cell death in cultures of oxidant exposed LMs. LMs from 7 lung fibrosis patients and healthy subjects were exposed to a physiologic concentration of H2O2 for 1 h. LMP was assessed with acridine orange green fluorescence, apoptosis/necrosis were estimated by apoptotic DNA and typical morphology, Fe was assessed with Prussian blue staining/atomic absorption spectrophotometry, and GSH was evaluated using a GSH assay kit. Oxidant-induced LMP and cell death were more pronounced in cultures of LMs from patients with lung fibrosis, and these cells contained less GSH and more cytochemically stained Fe. These observations indicate that LMP may be involved in fibrosis development, possibly through activation of the inflammasome complex. Further studies are warranted for a detailed understanding.

Place, publisher, year, edition, pages
Libertas Academica , 2013. Vol. 6, no 1, 69-74 p.
Keyword [en]
Apoptosis; Fibrosis; Inflammation; Iron; Macrophage
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-104950DOI: 10.4137/JCD.S13271OAI: diva2:700288
Available from: 2014-03-04 Created: 2014-03-04 Last updated: 2014-03-04

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Persson, Hans LennartVainikka, Linda
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Division of Cardiovascular MedicineFaculty of Health SciencesDepartment of Respiratory MedicineDivision of Cell Biology
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Journal of Cell Death
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