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Enzymatic Pretreatment of Lignocellulose Rich Waste for Improved Biogas Production
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The present study aimed to investigate the methane yield from anaerobic digestion of a lignocellulosic substrate subjected to different pretreatments. The lignocellulosic forest residues materials were milled and then pretreated with the organic solvent NMMO (N-Methylmorpholine N-oxide) and/or the lignolytic enzymes laccase and versatile peroxidase at a dosage of 60 U g-1 total solids (TS) substrate. The amount of methane produced was studied in a biomethane potential assay with inocula from a thermophilic biogas reactor treating municipal waste. All samples were run in triplicates. Due to the large amount of samples, two biomethane potential assays were conducted: series 10 & 20 and series 30 & 40. The gas production results show that NMMO-treated forest residues yielded 130 NmL CH4 g-1 volatile solids (VS) substrate and the untreated forest residues yielded 95 NmL CH4 g-1 VS substrate for series 10 & 20. For series 30 & 40, both untreated and NMMO-treated forest residues yielded 140 NmL CH4 g-1 VS substrate. NMMO-treatment appears to be favourable and no advantages from the enzyme pretreatment could be seen in terms of gas yield. An analysis of the reaction fluid after the enzymatic treatment showed presence of phenols, an indication of successful lignin hydrolysis.

Abstract [sv]

Studien avsåg att undersöka metanutbytet från anaerob nedbrytning med förbehandlad lignocellulosa som substrat. Lignocellulosamaterialet, i form av skogsavfall, maldes och förbehandlades därefter med det organiska lösningsmedlet NMMO (N-metylmorfolin-N-oxid) och/eller de lignolytiska enzymerna laccase och versatile peroxidas med dosen 60 U g-1 torrsubstanshalt (TS). Mängden producerad metan undersöktes i en biometanpotentialanalys med inocula från en termofil biogasreaktor, som behandlade hushållsavfall. Triplikat av varje prov användes för att öka den statistiska stabiliteten. På grund av det stora antalet prover genomfördes studien i två omgångar: Serie 10 & 20 samt serie 30 & 40. Resultaten visade att det NMMO-behandlade skogsavfallet gav 130 NmL CH4 g-1 organisk substans (VS) och det obehandlade skogsavfallet gav 95 NmL CH4 g-1 VS i serie 10 & 20. Både obehandlat och NMMO- behandlat skogsavfall gav 140 NmL CH4 g-1 VS i serie 30 & 40. Förbehandling med NMMO verkar vara fördelaktig medan enzymbehandling endast resulterade i en smärre ökning av gasproduktionen. En analys av vätskan efter enzymbehandlingen visade förekomst av fenoler, vilket visar på en lyckad ligninnedbrytning.

Place, publisher, year, edition, pages
2013. , 39 p.
Keyword [en]
Biogas, anaerobic digestion, lignin, laccase, versatile peroxidase, NMMO
Keyword [sv]
Biogas, anaerob nedbrytning, lignin, laccase, versatile peroxidas, NMMO
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-104974ISRN: TEMAV/TBM-EX--14/001--SEOAI: oai:DiVA.org:liu-104974DiVA: diva2:702868
External cooperation
Högskolan i Borås
Subject / course
Biotechnology
Presentation
2013-03-18, Linköping, 13:15 (Swedish)
Supervisors
Examiners
Available from: 2014-03-14 Created: 2014-03-04 Last updated: 2014-03-14Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
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  • Other style
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Language
  • de-DE
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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Output format
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