liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A High Precision Method for Quantitative Measurements of Reactive Oxygen Species in Frozen Biopsies
Norwegian University of Science and Technology, Trondheim, Norway.
Umeå University, Sweden.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology. Norwegian University of Science and Technology, Trondheim, Norway.
Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Biomedical Engineering.
2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 3Article in journal (Refereed) Published
Abstract [en]

Objective

An electron paramagnetic resonance (EPR) technique using the spin probe cyclic hydroxylamine 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetr​amethylpyrrolidine(CMH) was introduced as a versatile method for high precision quantification of reactive oxygen species, including the superoxide radical in frozen biological samples such as cell suspensions, blood or biopsies.

Materials and Methods

Loss of measurement precision and accuracy due to variations in sample size and shape were minimized by assembling the sample in a well-defined volume. Measurement was carried out at low temperature (150 K) using a nitrogen flow Dewar. The signal intensity was measured from the EPR 1st derivative amplitude, and related to a sample, 3-carboxy-proxyl (CP•) with known spin concentration.

Results

The absolute spin concentration could be quantified with a precision and accuracy better than ±10 µM (k = 1). The spin concentration of samples stored at −80°C could be reproduced after 6 months of storage well within the same error estimate.

Conclusion

The absolute spin concentration in wet biological samples such as biopsies, water solutions and cell cultures could be quantified with higher precision and accuracy than normally achievable using common techniques such as flat cells, tissue cells and various capillary tubes. In addition; biological samples could be collected and stored for future incubation with spin probe, and also further stored up to at least six months before EPR analysis, without loss of signal intensity. This opens for the possibility to store and transport incubated biological samples with known accuracy of the spin concentration over time.

Place, publisher, year, edition, pages
San Francisco: Public Library of Science , 2014. Vol. 9, no 3
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-105262DOI: 10.1371/journal.pone.0090964ISI: 000332483600116PubMedID: 24603936OAI: oai:DiVA.org:liu-105262DiVA: diva2:705058
Available from: 2014-03-14 Created: 2014-03-14 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(544 kB)136 downloads
File information
File name FULLTEXT01.pdfFile size 544 kBChecksum SHA-512
fe3b57ebc6964b1a771b5a57a8f687d4a00ff683fa289a60d8d133d3edc31098e55a5036ce90b5a2390411e797ccaaf4b3fa129fc11f715c390fe6c986216b90
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Lindgren, MikaelGustafsson, Håkan

Search in DiVA

By author/editor
Lindgren, MikaelGustafsson, Håkan
By organisation
ChemistryThe Institute of TechnologyDivision of Radiological SciencesFaculty of Health SciencesDepartment of Biomedical Engineering
In the same journal
PLoS ONE
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 136 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 180 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf