liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Methods for evaluating HER2 status in breast cancer: comparison of IHC, FISH, and real-time PCR analysis of formalin-fixed paraffin-embedded tissue
Linköping University, Department of Clinical and Experimental Medicine, Molecular and Immunological Pathology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Pathology and Clinical Genetics.
Linköping University, Department of Clinical and Experimental Medicine, Oncology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Oncology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Medical Genetics. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Pathology and Clinical Genetics.
2013 (English)In: Pathology and Laboratory Medicine International, ISSN 1179-2698, Vol. 5, 31-37 p.Article in journal (Refereed) Published
Abstract [en]

The human epidermal growth factor receptor 2 gene (HER2) is amplified in approximately 15%–20% of all breast cancers. This results in overexpression of the HER2 protein, which is associated with worse clinical outcomes in breast cancer patients. Several studies have shown that trastuzumab, a monoclonal antibody that interferes with the HER2/neu receptor, can improve overall survival in patients with HER2-positive breast cancer. Immunohistochemistry (IHC), combined with different methods for in situ hybridization, is currently used for routine assessment of HER2 status. The aim of the present study was to determine whether real-time polymerase chain reaction (PCR) can serve as a supplementary method for evaluation of HER2 status in primary breast cancer. For this purpose, 145 formalin-fixed paraffin-embedded primary breast cancer samples were tested by real-time PCR amplification of HER2, using amyloid precursor protein as a reference. The results were compared with HER2 status determined by fluorescence in situ hybridization (FISH) and IHC. The specificity, sensitivity, and reproducibility of real-time PCR were evaluated, and a comparison of formalin-fixed and fresh-frozen samples was performed. This showed concordance of 93% between real-time PCR and FISH, and 86% between real-time PCR and IHC. Therefore, we suggest that real-time PCR can be a useful supplementary method for assessment of HER2 status.

Place, publisher, year, edition, pages
Dove Medical Press , 2013. Vol. 5, 31-37 p.
Keyword [en]
17q, breast cancer, HER2, real-time PCR
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-105270DOI: 10.2147/PLMI.S44976OAI: oai:DiVA.org:liu-105270DiVA: diva2:705157
Available from: 2014-03-14 Created: 2014-03-14 Last updated: 2017-12-05Bibliographically approved

Open Access in DiVA

fulltext(610 kB)2029 downloads
File information
File name FULLTEXT01.pdfFile size 610 kBChecksum SHA-512
5886b28f03ef3e6e6b2a68003247a557fd3281b6f17b7e5476cc70dae90e2568148eace53743f6a7851671525d03eda0d2402114b693a7ef222d420207b73895
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Olsson, HansJansson, AgnetaHolmlund, BirgittaGunnarsson, Cecilia

Search in DiVA

By author/editor
Olsson, HansJansson, AgnetaHolmlund, BirgittaGunnarsson, Cecilia
By organisation
Molecular and Immunological PathologyFaculty of Health SciencesDepartment of Clinical Pathology and Clinical GeneticsOncologyMedical Genetics
In the same journal
Pathology and Laboratory Medicine International
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 2029 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 282 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf