liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
University of Helsinki, Finland .
University of Helsinki, Finland .
University of Helsinki, Finland .
University of Helsinki, Finland .
Show others and affiliations
2014 (English)In: BMC Genomics, ISSN 1471-2164, Vol. 15, no 129Article in journal (Refereed) Published
Abstract [en]

Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic groups (clades) of which clade 1 has shown extensive genome-wide introgression with C. jejuni, yet the other two clades (2 and 3) have less than 2% of C. jejuni ancestry. We characterized a C. coli strain (76339) with four novel multilocus sequence type alleles (ST-5088) and having the capability to express gamma-glutamyltranspeptidase (GGT); an accessory feature in C. jejuni. Our aim was to further characterize unintrogressed C. coli clades 2 and 3, using comparative genomics and with additional genome sequences available, to investigate the impact of horizontal gene transfer in shaping the accessory and core gene pools in unintrogressed C. coli. Results: Here, we present the first fully closed C. coli clade 3 genome (76339). The phylogenomic analysis of strain 76339, revealed that it belonged to clade 3 of unintrogressed C. coli. A more extensive respiratory metabolism among unintrogressed C. coli strains was found compared to introgressed C. coli (clade 1). We also identified other genes, such as serine proteases and an active sialyltransferase in the lipooligosaccharide locus, not present in C. coli clade 1 and we further propose a unique scenario for the evolution of Campylobacter ggt. Conclusions: We propose new insights into the evolution of the accessory genome of C. coli clade 3 and C. jejuni. Also, in silico analysis of the gene content revealed that C. coli clades 2 and 3 have genes associated with infection, suggesting they are a potent human pathogen, and may currently be underreported in human infections due to niche separation.

Place, publisher, year, edition, pages
BioMed Central , 2014. Vol. 15, no 129
Keyword [en]
Campylobacter coli; Comparative genomics; Phylogeny; Gamma glutamyltranspeptidase; Sialyltransferase
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-106035DOI: 10.1186/1471-2164-15-129ISI: 000332599600003OAI: diva2:712925
Available from: 2014-04-17 Created: 2014-04-17 Last updated: 2014-04-17

Open Access in DiVA

fulltext(1516 kB)305 downloads
File information
File name FULLTEXT01.pdfFile size 1516 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Schweda, Elke
By organisation
ChemistryThe Institute of Technology
In the same journal
BMC Genomics
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 305 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 34 hits
ReferencesLink to record
Permanent link

Direct link