liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Correlation between the effects of local and intracerebroventricular infusions of galanin on 5-HT release studied by microdialysis, and distribution of galanin and galanin receptors in prefrontal cortex, ventral hippocampus, amygdala, hypothalamus, and striatum of awake rats
Karolinska Institute, Stockholm, Sweden .
Karolinska Institute, Stockholm, Sweden and Atlas Antibodies AB, AlbaNova University Center, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden .
AstraZeneca R&D Montréal, Quebec, Canada.
Show others and affiliations
2014 (English)In: Synapse, ISSN 0887-4476, E-ISSN 1098-2396, Vol. 68, no 5, 179-193 p.Article in journal (Refereed) Published
Abstract [en]

The neuropeptide galanin is implicated in regulation of affective behavior, including modulation of 5-HT signaling. Here, we investigated, by use of microdialysis in freely moving rats, the effects of intracerebral (i.c.) and intracerebroventricular (i.c.v.) infusions of galanin on basal extracellular 5-HT levels in medial prefrontal cortex (mPFC), CA1 area of ventral hippocampus (vHPC), central amygdaloid nucleus (CeA), ventromedial hypothalamic nucleus ventrolateral part (VMHvl), and ventromedial caudate putamen (CPu). These results were compared with a parallel immunohistochemical analysis of the distribution of galanin, 5-HT, and noradrenaline (NA) nerve terminals, and with data on galanin receptors. Galanin i.c.v. significantly decreased the 5-HT levels in mPFC to 79% and in vHPC to 72%. Local infusions of galanin caused a long-lasting decrease in 5-HT levels in vHPC to 88%, and a moderate decrease in CeA, whereas the 5-HT levels in mPFC significantly increased to 121%. These effects of i.c. galanin correlated well with the density of 5-HT and galanin nerve terminals and galanin receptors autoradiography in mPFC, vHPC, and CeA. No effects of i.c. or i.c.v. galanin on 5-HT levels were observed in CPu or VMHvl, in agreement with the low numbers of galanin-positive terminals and low/moderate galanin receptor density. Galanin was often found to coexist in NA, but could never be detected in 5-HT terminals. Together the results show a neuroanatomical correlation between the effects of galanin infusions on 5-HT release and distribution of galanin and its receptors, and that i.c.v. and i.c. administration can give opposite effects on 5-HT release.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014. Vol. 68, no 5, 179-193 p.
Keyword [en]
caudate putamen; functional neuroanatomy; neuropeptides; noradrenaline; serotonin; transmitter coexistence
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-106012DOI: 10.1002/syn.21730ISI: 000332699900001PubMedID: 24430888OAI: diva2:712956
Available from: 2014-04-17 Created: 2014-04-17 Last updated: 2015-06-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Theodorsson, Elvar
By organisation
Division of Microbiology and Molecular MedicineFaculty of Health SciencesDepartment of Clinical Chemistry
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 205 hits
ReferencesLink to record
Permanent link

Direct link