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A miniaturized nanobiosensor for choline analysis
BITS, Goa, India.
Indian Institute of Technology Delhi, India.
Acromed Invest AB, Lund, Sweden.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0001-6235-7038
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2014 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 54, 558-564 p.Article in journal (Refereed) Published
Abstract [en]

A novel reusable chemiluminescence choline nanobiosensor has been developed using aligned zinc oxide nanorod-films (ZnONR). The chemically fashioned ZnONR were synthesized by hybrid wet chemical route onto glass substrates and used to fabricate a stable chemiluminescent choline biosensor. The biosensor was constructed by co-immobilization of the enzymes choline oxidase and peroxidase. The covalent immobilization of the enzymes on the ZnONR was achieved using 16-phosphonohexadecanoic acid as a cross-linker. The phosphonation of the ZnONR imparted significant stability to the immobilized enzyme as against physisorbed enzyme. A lower value of Michaelis-Menten constant (K-m), of 0.062 mM for the covalently coupled enzyme over the physisorbed enzymes facilitated enhanced stability of ZnONR nanobiosensor. The ZnONR-choline biosensor has been investigated over a wide range of choline from 0.0005 mM to 2 mM. Importantly, the recovery of choline in milk samples was close to 99%. Using the developed biosensor, choline was measurable even after 30 days with 60 repeated measurements proving the stability of the sensor (Intraday RSD%=2.83 and Interday RSD%=3.51).

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 54, 558-564 p.
Keyword [en]
Choline; Nanobiosensor; Zinc oxide nanorod; Chemiluminescence; Phosphonation; Milk
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-106116DOI: 10.1016/j.bios.2013.11.057ISI: 000333071500084OAI: diva2:714050
Available from: 2014-04-25 Created: 2014-04-24 Last updated: 2014-05-07Bibliographically approved

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Willander, Magnus
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