A miniaturized nanobiosensor for choline analysis
2014 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 54, 558-564 p.Article in journal (Refereed) Published
A novel reusable chemiluminescence choline nanobiosensor has been developed using aligned zinc oxide nanorod-films (ZnONR). The chemically fashioned ZnONR were synthesized by hybrid wet chemical route onto glass substrates and used to fabricate a stable chemiluminescent choline biosensor. The biosensor was constructed by co-immobilization of the enzymes choline oxidase and peroxidase. The covalent immobilization of the enzymes on the ZnONR was achieved using 16-phosphonohexadecanoic acid as a cross-linker. The phosphonation of the ZnONR imparted significant stability to the immobilized enzyme as against physisorbed enzyme. A lower value of Michaelis-Menten constant (K-m), of 0.062 mM for the covalently coupled enzyme over the physisorbed enzymes facilitated enhanced stability of ZnONR nanobiosensor. The ZnONR-choline biosensor has been investigated over a wide range of choline from 0.0005 mM to 2 mM. Importantly, the recovery of choline in milk samples was close to 99%. Using the developed biosensor, choline was measurable even after 30 days with 60 repeated measurements proving the stability of the sensor (Intraday RSD%=2.83 and Interday RSD%=3.51).
Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 54, 558-564 p.
Choline; Nanobiosensor; Zinc oxide nanorod; Chemiluminescence; Phosphonation; Milk
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-106116DOI: 10.1016/j.bios.2013.11.057ISI: 000333071500084OAI: oai:DiVA.org:liu-106116DiVA: diva2:714050