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Adsorption of Proteins at Solid Surfaces
Linköping University, Department of Physics, Chemistry and Biology, Applied Optics . Linköping University, The Institute of Technology.ORCID iD: 0000-0001-9229-2028
2014 (English)In: Ellipsometry of Functional Surfaces and Films / [ed] Hinrichs, Karsten; Eichhorn Klaus-Jochen, Springer Berlin/Heidelberg, 2014, 29-46 p.Chapter in book (Refereed)
Abstract [en]

Ellipsometry has a very high thin film sensitivity and can resolve sub-nm changes in the thickness of a protein film on a solid substrates. Being a technique based on photons in and photons out it can also be applied at solid-liquid interfaces. Ellipsometry has therefore found many in situ applications on protein layer dynamics but studies of protein layer structure are also frequent. Numerous ex situ applications on detection and quantification of protein layers are found and several biosensing concepts have been proposed. In this chapter, the use of ellipsometry in the above mentioned areas is reviewed and experimental methodology including cell design is briefly discussed. The classical ellipsometric challenge to determine both thickness and refractive index of a thin film is addressed and an overview of strategies to determine surface mass density is given. Included is also a discussion about spectral representations of optical properties of a protein layer in terms of a model dielectric function concept and its use for analysis of protein layer structure.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2014. 29-46 p.
, Springer Series in Surface Science, ISSN 0931-5195 ; 52
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-106409DOI: 10.1007/978-3-642-40128-2_2ISBN: 978-3-642-40127-5 (print)ISBN: 978-3-642-40128-2 (online)OAI: diva2:715924
Available from: 2014-05-07 Created: 2014-05-07 Last updated: 2014-05-16Bibliographically approved

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Arwin, Hans
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Applied Optics The Institute of Technology
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