Luminescent conjugated oligothiophenes: A novel dye for amyloid diagnostics
2013 (English)In: XIIIth International Symposium on Amyloidosis: From Misfolded Proteins to Well-Designed Treatment: The Proceedings of the XIIIth International Symposium on Amyloidosis,May 6-10, 2012, Groningen, The Netherlands / [ed] Bouke P.C. Hazenberg and Johan Bijzet, GUARD (Groningen Unit for Amyloidosis Research & Development) , 2013, 179-182 p.Conference paper (Refereed)
The alkaline Congo red staining method has, for almost half a century, been the gold standard of amyloid diagnosis. Unfortunately, the method is both laborious and requires great skill to achieve proper diagnosis. In this study we are presenting an alternative method that is compatible with immunofluorescence typing. We used a novel dye, h-FTAA, designed and synthesized by us. The dye belongs to the novel class of conformation sensitive dyes known as Luminescent conjugated oligothiophenes (LCOs). We examined 37 different cases of systemic amyloidoses from various tissues. It was found that h-FTAA binds to amyloid with higher sensitivity and greater selectivity than Congo red, as was determined by both fluorescence- and light polarization microscopy. Due to the methods ease of use and performance compared to Congo red, it is concluded that h-FTAA is a better first choice for screening of systemic amyloidoses.
Place, publisher, year, edition, pages
GUARD (Groningen Unit for Amyloidosis Research & Development) , 2013. 179-182 p.
Chemical Sciences Natural Sciences
IdentifiersURN: urn:nbn:se:liu:diva-106789ISBN: 978-90-821593-0-1 (print)ISBN: 978-90-821593-1-8 (ebook)OAI: oai:DiVA.org:liu-106789DiVA: diva2:719117
The XIIIth International Symposium on Amyloidosis, May 6-10, 2012, Groningen, The Netherlands