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miR-20b regulates expression of proteinase-activated receptor-1 (PAR-1) thrombin receptor in melanoma cells
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences.
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2014 (English)In: Pigment Cell & Melanoma Research, ISSN 1755-1471, E-ISSN 1755-148X, Vol. 27, no 3, 431-441 p.Article in journal (Refereed) Published
Abstract [en]

The proteinase-activated receptor 1 (PAR-1) plays a central role in melanoma progression and its expression level is believed to correlate with the degree of cancer invasiveness. Here, we show that PAR-1 is post-transcriptionally regulated by miR-20b microRNA in human melanoma cells. PAR-1 was found to be expressed in metastatic melanoma cells but was barely detectable in primary melanoma. By transducing primary melanoma cells with a lentivirus containing a 3-UTR construct of PAR-1 mRNA, we could show that endogenous melanoma microRNAs interacted with PAR-1 3-UTR and silenced a fused luciferase reporter. Transfection of an inhibitor against miR-20b into primary melanoma cells reversed this process. Finally, transfection of miR-20b mimic into metastatic melanoma cells caused downregulation of the luciferase reporter. We conclude that miR-20b regulates expression of melanoma PAR-1 receptor, which may explain the differential expression of PAR-1 observed in human melanoma.

Place, publisher, year, edition, pages
Wiley , 2014. Vol. 27, no 3, 431-441 p.
Keyword [en]
melanoma; metastasis; proteinase-activated receptor-1; gene expression regulation; microRNAs
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-106844DOI: 10.1111/pcmr.12217ISI: 000334170900014OAI: oai:DiVA.org:liu-106844DiVA: diva2:720131
Available from: 2014-05-28 Created: 2014-05-23 Last updated: 2017-12-05

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Faxälv, LarsClaesson, KjerstiJönsson, Jan-IngvarOsman, Abdimajid

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Faxälv, LarsClaesson, KjerstiJönsson, Jan-IngvarOsman, Abdimajid
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Pigment Cell & Melanoma Research
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