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Ovariectomy and 17β-estradiol replacement in rats and mice: a visual demonstration
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Anaesthetics, Operations and Specialty Surgery Center, Department of Neurosurgery.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences.ORCID iD: 0000-0001-8813-0384
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences.
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2012 (English)In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, no 64, 4013- p.Article in journal (Refereed) Published
Abstract [en]

Estrogens are a family of female sexual hormones with an exceptionally wide spectrum of effects. When rats and mice are used in estrogen research they are commonly ovariectomized in order to ablate the rapidly cycling hormone production, replacing the 17β-estradiol exogenously. There is, however, lack of consensus regarding how the hormone should be administered to obtain physiological serum concentrations This is crucial since the 17β-estradiol level/administration method profoundly influences the experimental results1-3. We have in a series of studies characterized the different modes of 17β-estradiol administration, finding that subcutaneous silastic capsules and per-oral nut-cream Nutella are superior to commercially available slow-release pellets (produced by the company Innovative Research of America) and daily injections in terms of producing physiological serum concentrations of 17β-estradiol4-6. Amongst the advantages of the nut-cream method, that previously has been used for buprenorphine administration7, is that when used for estrogen administration it resembles peroral hormone replacement therapy and is non-invasive. The subcutaneous silastic capsules are convenient and produce the most stable serum concentrations. This video article contains step-by-step demonstrations of ovariectomy and 17β-estradiol hormone replacement by silastic capsules and peroral Nutella in rats and mice, followed by a discussion of important aspects of the administration procedures.

Place, publisher, year, edition, pages
Journal of Visualized Experiments , 2012. no 64, 4013- p.
Keyword [en]
Medicine, Issue 64, Physiology, Oophorectomy, Rat, Mouse, 17β-estradiol, Administration, Silastic capsules, Nutella
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-107098DOI: 10.3791/4013ISI: 000209223100035PubMedID: 22710371OAI: oai:DiVA.org:liu-107098DiVA: diva2:721882
Available from: 2014-06-05 Created: 2014-06-05 Last updated: 2017-12-05Bibliographically approved

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Ström, Jakob OTheodorsson, AnnetteIngberg, Edvin

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Clinical ChemistryFaculty of Health SciencesDepartment of NeurosurgeryDepartment of Clinical Chemistry
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