Comparison of different sorbent materials for solid-phase extraction of selected drugs in human urine analyzed by UHPLC-UV
2014 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 958, 22-28 p.Article in journal (Refereed) Published
A procedure based on solid-phase extraction (SPE) followed by ultra-high-performance liquid chromatography (UHPLC) with UV detection has been developed for the analysis of multiple drugs in human urine. The compounds evaluated were aliskiren, prasugrel, rivaroxaban, prednisolone, propranolol, ketoprofen, nifedipine, naproxen, terbinafine, ibuprofen, diclofenac, sildenafil and acenocoumarol. Seventeen different solid phase extraction (SPE) cartridges were tested to evaluate their applicability for the isolation of drugs from human urine. Comparison were recovery of different drugs and reproducibility. The samples were analyzed by UHPLC using a Poroshell 120 EC-C18 column and acetonitrile -0.05% TFA in water as the mobile phase under gradient elution conditions. SPE combined with UHPLC UV allowed the determination of drugs over a linear range of 0.01-30.0 mu g/mL, with limits of detection at 0.003-0.217 mu g/mL and precision of 0.8-7.1%. Phenyl (C6H5) sorbent was found to provide the most effective clean-up, removing the greatest amount of interfering substance and simultaneously ensuring analyte recoveries higher than 85.5% with relative standard deviations (RSD)less than10%. The method was applied with good accuracy and precision in the determination of drugs in human urine obtained from patients treated with selected drugs.
Place, publisher, year, edition, pages
Elsevier , 2014. Vol. 958, 22-28 p.
Sorbent comparison; Solid-phase extraction; Drugs; Liquid chromatography
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-107118DOI: 10.1016/j.jchromb.2014.03.014ISI: 000335630200004OAI: oai:DiVA.org:liu-107118DiVA: diva2:722022