New astrovirus in human feces from Burkina Faso
2014 (English)In: Journal of Clinical Virology, ISSN 1386-6532, E-ISSN 1873-5967, Vol. 60, no 2, 161-164 p.Article in journal (Refereed) Published
Background: A significant fraction of cases of diarrhea, a leading cause of childhood mortality worldwide, remain unexplained. Objectives: To identify viruses in unexplained cases of diarrhea using an unbiased metagenomics approach. Study design: Viral nucleic acids were enriched from the feces from 48 cases of unexplained diarrhea from Burkina Faso, sequenced, and compared against all known viral genomes. Results: The full genome of a highly divergent astrovirus was sequenced in a sample co-infected with parechovirus 1. RT-PCR identified a single astrovirus infection in these 48 patients indicating a low prevalence. Human astrovirus-BF34 was most closely related to mamastrovirus species 8 and 9 also found in human with which it shared 62%, 74%, and 57% amino acid identities over its protease, RNA dependent RNA polymerase and capsid proteins, respectively. Conclusions: Burkina Faso astrovirus is proposed as prototype for a novel species in the genus Mamastrovirus, here tentatively called Mamastrovirus 20, representing the fifth human astrovirus species.
Place, publisher, year, edition, pages
Elsevier , 2014. Vol. 60, no 2, 161-164 p.
Astrovirus; Species; Diarrhea; Metagenomics; Children
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-107443DOI: 10.1016/j.jcv.2014.03.024ISI: 000335738000013OAI: oai:DiVA.org:liu-107443DiVA: diva2:724335