Self-Reporting Micellar Polymer Nanostructures for Optical Urea Biosensing
2014 (English)In: Industrial & Engineering Chemistry Research, ISSN 0888-5885, E-ISSN 1520-5045, Vol. 53, no 20, 8509-8514 p.Article in journal (Refereed) Published
We report the facile fabrication of a self-reporting, highly sensitive and selective optical urea nanobiosensor using chitosan-g-polypyrrole (CHIT-g-PPy) nanomicelles as a sensing platform. Urease was immobilized on the spherical micellar surface to create an ultrasensitive self-reporting nanobiosystem for urea. The resulting nanostructures show monodisperse size distributions before and after enzyme loading. The critical micelle concentration of the enzyme-immobilized polymer nanostructure was measured to be 0.49 mg/L in phosphate buffer at pH 7.4. The nanobiosensor had a linear optical response to urea concentrations ranging from 0.01 to 30 mM with a response time of a few seconds. This promising approach provides a novel methodology for self-reporting bioassembly over nanostructure polymer micelles and furnishes the basis for fabrication of sensitive and efficient optical nanobiosensors.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2014. Vol. 53, no 20, 8509-8514 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-107837DOI: 10.1021/ie5012799ISI: 000336417700022OAI: oai:DiVA.org:liu-107837DiVA: diva2:727905