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Self-Reporting Micellar Polymer Nanostructures for Optical Urea Biosensing
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. University of Delhi, India .
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, Faculty of Science & Engineering. University of Delhi, India .
University of Delhi, India .
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2014 (English)In: Industrial & Engineering Chemistry Research, ISSN 0888-5885, E-ISSN 1520-5045, Vol. 53, no 20, 8509-8514 p.Article in journal (Refereed) Published
Abstract [en]

We report the facile fabrication of a self-reporting, highly sensitive and selective optical urea nanobiosensor using chitosan-g-polypyrrole (CHIT-g-PPy) nanomicelles as a sensing platform. Urease was immobilized on the spherical micellar surface to create an ultrasensitive self-reporting nanobiosystem for urea. The resulting nanostructures show monodisperse size distributions before and after enzyme loading. The critical micelle concentration of the enzyme-immobilized polymer nanostructure was measured to be 0.49 mg/L in phosphate buffer at pH 7.4. The nanobiosensor had a linear optical response to urea concentrations ranging from 0.01 to 30 mM with a response time of a few seconds. This promising approach provides a novel methodology for self-reporting bioassembly over nanostructure polymer micelles and furnishes the basis for fabrication of sensitive and efficient optical nanobiosensors.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2014. Vol. 53, no 20, 8509-8514 p.
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Engineering and Technology
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URN: urn:nbn:se:liu:diva-107837DOI: 10.1021/ie5012799ISI: 000336417700022OAI: oai:DiVA.org:liu-107837DiVA: diva2:727905
Available from: 2014-06-23 Created: 2014-06-23 Last updated: 2017-12-05Bibliographically approved

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Parlak, OnurTurner, AnthonyTiwari, Ashutosh

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