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Segmental analysis of amphetamines in hair using a sensitive UHPLC-MS/MS method
Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Linköping, Sweden.
Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Health Sciences. Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Linköping, Sweden.
2014 (English)In: Drug Testing and Analysis, ISSN 1942-7603, E-ISSN 1942-7611, Vol. 6, 22-29 p.Article in journal (Refereed) Published
Abstract [en]

A sensitive and robust ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxy methamphetamine in hair samples. Segmented hair (10 mg) was incubated in 2M sodium hydroxide (80 degrees C, 10 min) before liquid-liquid extraction with isooctane followed by centrifugation and evaporation of the organic phase to dryness. The residue was reconstituted in methanol:formate buffer pH 3 (20:80). The total run time was 4 min and after optimization of UHPLC-MS/MS-parameters validation included selectivity, matrix effects, recovery, process efficiency, calibration model and range, lower limit of quantification, precision and bias. The calibration curve ranged from 0.02 to 12.5 ng/mg, and the recovery was between 62 and 83%. During validation the bias was less than +/- 7% and the imprecision was less than 5% for all analytes. In routine analysis, fortified control samples demonstrated an imprecision less than13% and control samples made from authentic hair demonstrated an imprecision less than26%. The method was applied to samples from a controlled study of amphetamine intake as well as forensic hair samples previously analyzed with an ultra high performance liquid chromatography time of flight mass spectrometry (UHPLC-TOF-MS) screening method. The proposed method was suitable for quantification of these drugs in forensic cases including violent crimes, autopsy cases, drug testing and re-granting of driving licences. This study also demonstrated that if hair samples are divided into several short segments, the time point for intake of a small dose of amphetamine can be estimated, which might be useful when drug facilitated crimes are investigated.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014. Vol. 6, 22-29 p.
Keyword [en]
forensic; segmental analysis; hair; LC-MS-MS; amphetamines
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-107826DOI: 10.1002/dta.1637ISI: 000335632900006OAI: diva2:727936
Available from: 2014-06-23 Created: 2014-06-23 Last updated: 2014-11-25Bibliographically approved

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Kronstrand, Robert
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