Segmental analysis of amphetamines in hair using a sensitive UHPLC-MS/MS method
2014 (English)In: Drug Testing and Analysis, ISSN 1942-7603, E-ISSN 1942-7611, Vol. 6, 22-29 p.Article in journal (Refereed) Published
A sensitive and robust ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for quantification of amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine and 3,4-methylenedioxy methamphetamine in hair samples. Segmented hair (10 mg) was incubated in 2M sodium hydroxide (80 degrees C, 10 min) before liquid-liquid extraction with isooctane followed by centrifugation and evaporation of the organic phase to dryness. The residue was reconstituted in methanol:formate buffer pH 3 (20:80). The total run time was 4 min and after optimization of UHPLC-MS/MS-parameters validation included selectivity, matrix effects, recovery, process efficiency, calibration model and range, lower limit of quantification, precision and bias. The calibration curve ranged from 0.02 to 12.5 ng/mg, and the recovery was between 62 and 83%. During validation the bias was less than +/- 7% and the imprecision was less than 5% for all analytes. In routine analysis, fortified control samples demonstrated an imprecision less than13% and control samples made from authentic hair demonstrated an imprecision less than26%. The method was applied to samples from a controlled study of amphetamine intake as well as forensic hair samples previously analyzed with an ultra high performance liquid chromatography time of flight mass spectrometry (UHPLC-TOF-MS) screening method. The proposed method was suitable for quantification of these drugs in forensic cases including violent crimes, autopsy cases, drug testing and re-granting of driving licences. This study also demonstrated that if hair samples are divided into several short segments, the time point for intake of a small dose of amphetamine can be estimated, which might be useful when drug facilitated crimes are investigated.
Place, publisher, year, edition, pages
John Wiley & Sons, 2014. Vol. 6, 22-29 p.
forensic; segmental analysis; hair; LC-MS-MS; amphetamines
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-107826DOI: 10.1002/dta.1637ISI: 000335632900006OAI: oai:DiVA.org:liu-107826DiVA: diva2:727936