Hybrid Nanoparticle-Liposome Detection of Phospholipase Activity
2011 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 11, no 4, 1401-1405 p.Article in journal (Refereed) Published
A flexible nanoparticle-based phospholipase (PL) assay is demonstrated in which the enzymatic substrate is decoupled from the nanoparticle surface. Liposomes are loaded with a polypeptide that is designed to heteroassociate with a second polypeptide immobilized on gold nanoparticies. Release of this polypeptide from the liposornes, triggered by PL, induces a folding-dependent nanoparticle bridging aggregation. The colorimetric response from this aggregation enables straightforward and continuous detection of PL in the picomolar range. The speed, specificity, and flexibility of this assay make it appropriate for a range of applications, from point of care diagnostics to high throughput pharmaceutical screening.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2011. Vol. 11, no 4, 1401-1405 p.
Phospholipase A2, gold nanoparticles, liposomes, polypeptides, helix-loop-helix, functionalized gold nanoparticles, activity, colorimetric detection, vesicles, hydrolysis, mechanism
IdentifiersURN: urn:nbn:se:liu:diva-107949DOI: 10.1021/nl1024062ISI: 000289341500001OAI: oai:DiVA.org:liu-107949DiVA: diva2:728270