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Electrochemical evaluation of troponin T imprinted polymer receptor
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. Ferdowsi University of Mashhad, Mashhad, Iran.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-1815-9699
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
2014 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 59, 160-165 p.Article in journal (Refereed) Published
Abstract [en]

The selective detection and quantification of macromolecular targets is a fundamental biological mechanism in nature. Molecularly imprinted polymers (MIPs) have been identified as one of the most promising synthetic alternatives to bioreceptors. However, expanding this methodology towards selective recognition of bulky templates such as proteins appears to be extremely challenging due to problems associated with removal of the template from the polymeric network. In this study, polymer imprinted with troponin T (TnT) was assessed using electrochemical methods and the influence of various extraction methods, including conventional immersion extraction, thermal annealing and ultrasonic-assisted extraction, on the binding characteristics of the troponin-to-imprinted polymer receptor was elucidated. Cyclic voltammetric deposition of o-phenylenediamine (o-PD) film in the presence of TnT as a template was performed in acetate buffer (0.5 M, pH 5.2) on a gold substrate. Solvent extraction of the target molecule was optimised and followed by subsequent washing with water. The electrochemistry of a ferro/ferricyanide probe was used to characterise the TnT MIP receptor film. The incubation of the TnT MIP receptor-modified electrode with respect to TnT concentration resulted in a suppression of the ferro/ferricyanide redox current. The dissociation constant (KD) was calculated using a two-site model of template affinity for the TnT MIP receptor. The synthetic TnT MIP receptor had high affinity for TnT with a KD of 2.3×10−13 M.

Place, publisher, year, edition, pages
USA: Elsevier, 2014. Vol. 59, 160-165 p.
Keyword [en]
Synthetic receptor; Imprinted polymer; Troponin T; Template affinity; Electrochemical study
National Category
Medical Materials
Identifiers
URN: urn:nbn:se:liu:diva-107967DOI: 10.1016/j.bios.2014.03.013ISI: 000337855100025OAI: oai:DiVA.org:liu-107967DiVA: diva2:728440
Projects
European Commission, Belgium (PIIFGA-2009-254955), LIST, Sweden, Ferdowsi University of Mashhad, Iran (Code: 15616/3, dated: 2010)
Funder
EU, FP7, Seventh Framework Programme, PIIFGA-2009-254955
Available from: 2014-06-24 Created: 2014-06-24 Last updated: 2017-12-05Bibliographically approved

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Turner, Anthony P.F.Tiwari, Ashutosh

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