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Ultrasensitive detection of human liver hepatocellular carcinoma (HepG2) cells using a label-free aptasensor
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. University of Isfahan, Iran.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology. University of Isfahan, Iran.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-1815-9699
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2014 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 86, no 10, 4956-4960 p.Article in journal (Refereed) Published
Abstract [en]

Liver cancer is one of the most common cancers in the world and has no effective cure, especially in later stages. The development of a tangible protocol for early diagnosis of this disease remains a major challenge. In the present manuscript, an aptamer-based, label-free electrochemical biosensor for the sensitive detection of HepG2, a hepatocellular carcinoma cell line, is described. The target cells are captured in a sandwich architecture using TLS11a aptamer covalently attached to a gold surface and a secondary TLS11a aptamer. The application of TLS11a aptamer as a recognition layer resulted in a sensor with high affinity for HepG2 cancer cells in comparison with control cancer cells of human prostate, breast and colon tumours. The aptasensor delivered a wide linear dynamic range over 1 × 102 – 1 × 106 cell/mL, with a detection limit of 2 cell/mL. This protocol provides a precise method for sensitive detection of liver cancer with significant advantages in terms of simplicity, low cost, and stability.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2014. Vol. 86, no 10, 4956-4960 p.
Keyword [en]
Hepatocellular carcinoma, cancer cells, aptamer, electrochemical aptasensor, label-free.
National Category
Medical Engineering
Identifiers
URN: urn:nbn:se:liu:diva-107976DOI: 10.1021/ac500375pISI: 000336418600043OAI: oai:DiVA.org:liu-107976DiVA: diva2:728459
Projects
European Commission (PIIF-GA-2009-254955), IGEN and LIST, Linköping University
Funder
EU, FP7, Seventh Framework Programme, PIIF-GA-2009-254955
Available from: 2014-06-24 Created: 2014-06-24 Last updated: 2017-12-05Bibliographically approved

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Mehrgardi, MasoudWiechec, EmiliaTurner, Anthony P.F.Tiwari, Ashutosh

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