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Optical biosensors based on universal pH indicator as a reporter for quantification of clinically-relevant compounds
University of Leicester, UK.
Cranfield University, Bedfordshire, UK.
Cranfield University, Bedfordshire, UK.ORCID iD: 0000-0002-1815-9699
Nitto Denko Asia Technical Centre PTE Ltd., Synapse, Singapore.
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2014 (English)In: Journal of the Chinese Advanced Materials Society, ISSN 2168-1031, Vol. 2, no 2, 99-109 p.Article in journal (Refereed) Published
Abstract [en]

It was shown that universal indicator, a blend of pH-sensitive dyes which changes colour from red to purple over broad pH value range, in combination with a disposable optical sensor chip, could be used for the monitoring of physiologically important compounds. Changes in the optical properties of pH-sensitive dyes in the presence of enzyme catalysed reactions allowed quick and sensitive evaluation of the concentration of clinically important analytes such as glucose and urea. It was shown that the proposed optical biosensor could provide a sensitive and inexpensive method for the quantification of urea in plasma over a concentration range of 5–50 mM and glucose in the concentration range 1–25 mM. The system provides a generic platform that can be combined with other appropriate enzymes for the quantification of a range of physiological compounds to furnish versatile and portable clinical multisensors.

Place, publisher, year, edition, pages
Taylor & Francis, 2014. Vol. 2, no 2, 99-109 p.
Keyword [en]
universal indicator, optical sensor, point of care testing, glucose oxidase, multisensor
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URN: urn:nbn:se:liu:diva-108408DOI: 10.1080/22243682.2014.910474OAI: oai:DiVA.org:liu-108408DiVA: diva2:730233
Available from: 2014-06-27 Created: 2014-06-27 Last updated: 2017-07-12Bibliographically approved

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Turner, Anthony

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