On/off-switchable folic acid sensor using molecularly imprinted smart polymer electrode
2014 (English)In: 24thAnniversary World Congress on Biosensors – Biosensors 2014, Elsevier, 2014Conference paper, Abstract (Other academic)
Recently, much attention has been focused on the development of controlled switchable surfaces, also known as “smart surfaces”, which switch their physicochemical properties in response to external stimuli . Switching of a surface based on temperature can be realised using thermo-sensitive polymers, which undergo a phase transition at the lower critical solution temperature (LCST), where their behavior switches between hydrophobic and hydrophilic . LCST modulation can be achieved by copolymerisation with other monomers in order to produce a LCST close to physiological temperature. Thus, it could be useful in controllable, temperature-responsive bio-switches for biomedical and biotechnology applications . The combination of smart polymers with molecular imprinting offers a powerful tool to design more effective sensors and medical devices. In this study, a temperature sensitive amine-terminated poly(N-isopropylacrylamide) block with (N,N'-methylenebisacrylamide) cross-linker along with o-phenylenediamine was electropolymerised on a gold electrode in the presence of folic acid (FA) as template to produce an on/off-switchable molecularly imprinted polymer (MIP) affinity sensor for folic acid. Differential pulse voltammetry and cyclic voltammetry were used to characterise the FA-imprinted layer. Incubation of the MIP-modified electrode with FA resulted in a suppression of the ferro/ferricyanide redox process. The highest sensitivity of this temperature gated on/off-switchable folic acid sensor was achieved at 22 ºC. Such switchable affinity materials offer considerable potential for the design of highly selective and controllable biosensors and immunoassays.
Place, publisher, year, edition, pages
IdentifiersURN: urn:nbn:se:liu:diva-108424OAI: oai:DiVA.org:liu-108424DiVA: diva2:730282
24th Anniversary World Congress on Biosensors – Biosensors 2014, 27-30 May 2014, Melbourne, Australia