Label-free Detection of Prostate Cancer Biomarker
2014 (English)In: 24th Anniversary World Congress on Biosensors – Biosensors 2014 / [ed] Anthony Turner, Elsevier, 2014Conference paper, Poster (Other academic)
Prostate cancer is common and a frequent cause of cancer death, especially for American men. Most prostate cancers progress very slowly and while on the average about 30% of American males develop prostate cancer, only 3% die from the disease. The disparity in the statistics indicates that a more effective screening method is required to differentiate the aggressive form of prostate cancer that causes mortality in patients. In this work, a label-free, novel microelectromechanical (MEMS) biosensor for detection of cysteine, a prostate cancer biomarker is presented. This biosensor merges two biosensing techniques, namely resonant frequency measurements and electrochemical impedance spectroscopy (EIS) on a single biosensor. The sensor is based on the innovative placement of the working microelectrodes for EIS technique as the top electrode of a quartz crystal microbalance (QCM) resonator. The QCM acoustic wave sensor consists of a thin AT-cut quartz substrate with two gold electrodes on both sides. The top metal electrode used for generating the acoustic wave is also used for EIS measurements of the biosensor, as illustrated in Fig. 1. The surfaces of the gold electrodes are modified using molecularly-imprinted polymers. Electrochemical methods were used to monitor the self-assembly of cysteine on the gold surface. The thiol-gold self-assembled layer causes impedance and mass change, which was characterised by EIS and acoustic-wave methods, respectively. Preliminary tests were performed on this sensor using cysteine in both phosphate buffer saline and human serum.
Place, publisher, year, edition, pages
IdentifiersURN: urn:nbn:se:liu:diva-108427OAI: oai:DiVA.org:liu-108427DiVA: diva2:730287
24th Anniversary World Congress on Biosensors – Biosensors 2014, 27-30 May 2014, Melbourne, Australia